論文 - 詳細
| RRC ID | 86777 |
|---|---|
| 著者 | Otsuna H, Hutcheson DA, Duncan RN, McPherson AD, Scoresby AN, Gaynes BF, Tong Z, Fujimoto E, Kwan KM, Chien CB, Dorsky RI. |
| タイトル | High-resolution analysis of central nervous system expression patterns in zebrafish Gal4 enhancer-trap lines. |
| ジャーナル | Dev Dyn |
| Abstract |
BACKGROUND:The application of the Gal4/UAS system to enhancer and gene trapping screens in zebrafish has greatly increased the ability to label and manipulate cell populations in multiple tissues, including the central nervous system (CNS). However the ability to select existing lines for specific applications has been limited by the lack of detailed expression analysis. RESULTS:We describe a Gal4 enhancer trap screen in which we used advanced image analysis, including three-dimensional confocal reconstructions and documentation of expression patterns at multiple developmental time points. In all, we have created and annotated 98 lines exhibiting a wide range of expression patterns, most of which include CNS expression. Expression was also observed in nonneural tissues such as muscle, skin epithelium, vasculature, and neural crest derivatives. All lines and data are publicly available from the Zebrafish International Research Center (ZIRC) from the Zebrafish Model Organism Database (ZFIN). CONCLUSIONS:Our detailed documentation of expression patterns, combined with the public availability of images and fish lines, provides a valuable resource for researchers wishing to study CNS development and function in zebrafish. Our data also suggest that many existing enhancer trap lines may have previously uncharacterized expression in multiple tissues and cell types. |
| 巻・号 | 244(6) |
| ページ | 785-96 |
| 公開日 | 2015-6-1 |
| DOI | 10.1002/dvdy.24260 |
| PMID | 25694140 |
| PMC | PMC4449297 |
| MeSH | Animals Animals, Genetically Modified / embryology Animals, Genetically Modified / genetics* Central Nervous System / embryology Central Nervous System / metabolism* DNA Transposable Elements DNA-Binding Proteins / genetics* Databases, Factual Enhancer Elements, Genetic* Gene Expression Profiling / methods* Gene Expression Regulation, Developmental* Genes, Reporter* Genes, Synthetic Imaging, Three-Dimensional / methods* Luminescent Proteins / analysis Luminescent Proteins / genetics Mutagenesis, Insertional Nerve Tissue Proteins / biosynthesis Nerve Tissue Proteins / genetics* Neurons / metabolism Organ Specificity Red Fluorescent Protein Saccharomyces cerevisiae Proteins / genetics* Transcription Factors / genetics* Zebrafish / embryology Zebrafish / genetics* Zebrafish Proteins / biosynthesis Zebrafish Proteins / genetics* |
| IF | 3.275 |
| リソース情報 | |
| ゼブラフィッシュ | UAS:GFP |