| Abstract |
Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder. This study aims to investigate the expression of miR-103a-3p in PCOS and its potential molecular mechanisms. RT-qPCR was used to detect the levels of miR-103a-3p in the serum of PCOS patients, and to analyze its correlation with clinical indicators. The diagnostic value of miR-103a-3p for PCOS was evaluated through the ROC curve. Subsequently, the viability of KGN cells, inflammatory factors, and oxidative stress indicators were detected using CCK-8, ELISA, and oxidative stress detection kits, respectively. Finally, the targeting relationship between miR-103a-3p and PTEN was validated using dual luciferase reporter and RIP assays. The expression of miR-103a-3p in the serum of PCOS patients was significantly lower than that in the control group. miR-103a-3p demonstrates high sensitivity and specificity for the diagnosis of PCOS (AUC = 0.881). The expression level of miR-103a-3p is correlated with clinical indicators. In KGN cells, overexpression of miR-103a-3p significantly enhances cell viability, inhibits the secretion of inflammatory factors, and reduces oxidative stress levels, whereas inhibition of miR-103a-3p exhibits the opposite effects. The dual luciferase reporter assay and RIP experiment confirmed the direct interaction between miR-103a-3p and PTEN. In PCOS patients, miR-103a-3p is expressed at low levels and is closely associated with hormonal and metabolic indicators, potentially serving as an early diagnostic marker for PCOS. In vitro studies have found that miR-103a-3p may inhibit the progression of PCOS inflammation by targeting PTEN.
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