論文 - 詳細
| RRC ID | 86860 |
|---|---|
| 著者 | Norizuki T, Kushida Y, Sekimoto T, Sasaki T, Yamano K, Matsuda N, Sasaki R, Noda NN, Sato K, Sato M. |
| タイトル | ALLO-1a is a ubiquitin-binding adaptor for allophagy in Caenorhabditis elegans. |
| ジャーナル | J Cell Sci |
| Abstract |
In the nematode Caenorhabditis elegans, sperm-derived mitochondria and membranous organelles (MOs) are selectively degraded by autophagy in embryos in a process termed allophagy. For this process, ALLO-1 functions as an autophagy adaptor. The allo-1 gene encodes two splice isoforms, ALLO-1a and ALLO-1b, which have different C-terminal sequences and are predominantly targeted to MOs and paternal mitochondria, respectively. However, the mechanism by which ALLO-1 targets the paternal organelles remains unknown. In this study, X-ray crystallography analysis reveals that the C-terminal region of ALLO-1a forms a parallel coiled-coil structure. In addition, AlphaFold2-Multimer predicts that this region directly interacts with ubiquitin. We showed that ALLO-1a interacts with K48- and K63-linked polyubiquitin in vitro and found that the D355 residue of ALLO-1a at the predicted interface with ubiquitin is important for its ubiquitin binding in vitro and also for its MO targeting and MO degradation in embryos. These results suggest that ubiquitin is a marker for the recognition of MOs by the autophagy machinery in C. elegans embryos. |
| 巻・号 | 138(24) |
| 公開日 | 2025-12-15 |
| DOI | 10.1242/jcs.264252 |
| PII | 369838 |
| PMID | 41234204 |
| MeSH | Animals Autophagy* Caenorhabditis elegans* / embryology Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / chemistry Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Embryo, Nonmammalian / metabolism Mitochondria / metabolism Protein Binding Ubiquitin* / metabolism |
| IF | 4.573 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 18 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.2 |
| リソース情報 | |
| 遺伝子材料 | CSII-CMV-MCS-IRES2-Bsd (RDB04385) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) |