Reference - Detail
| RRC ID | 86869 |
|---|---|
| Author | Labandera AM, Toth R, Mitchell S, Johnson JJ, Kurucz B, Puyaubert J, Baudouin E, Uhrig RG, Moorhead GB. |
| Title | Phospho-Proteomics Identifies D-Group MAP Kinases as Substrates of the Arabidopsis Tyrosine Phosphatase RLPH2. |
| Journal | Plant Direct |
| Abstract |
Despite being one of the few bona fide plant tyrosine phosphatases, the Arabidopsis thalianaRhizobiales-like phosphatase 2 (RLPH2) has no known substrates. Utilizing phospho-proteomics, we identified the activation loop phospho-tyrosine of several A. thaliana D-group mitogen-activated protein kinases (MPKs) as potential RLPH2 substrates. All Arabidopsis D-group MPKs possess a TDY activation loop phosphorylation motif, whereas other MPKs (Groups A, B, and C) contain a TEY motif. Our findings reveal that RLPH2 has a strong preference for aspartate (D) in the TXY motif, providing specificity for RLPH2 to exclusively target and dephosphorylate the D-group MPKs. Additionally, D-group MPKs contain a unique activation loop insertion that conforms to a protein phosphatase one (PP1) binding motif, with findings presented here confirming Arabidopsis PP1 phosphatases dock at this site. Intriguingly, only D-group MPKs among all identified Arabidopsis protein kinases possess this PP1 recruiting motif. Using multiple RLPH2-deficient plant lines, we demonstrate that RLPH2 represses seed dormancy release. Overall, this work highlights the power of phospho-proteomics in identifying substrates of this novel plant tyrosine phosphatase while also revealing new complexities in the interactions between MPK activation loops and multiple phospho-mediated cell signaling events. |
| Volume | 10(1) |
| Pages | e70137 |
| Published | 2026-1-1 |
| DOI | 10.1002/pld3.70137 |
| PII | PLD370137 |
| PMID | 41567881 |
| PMC | PMC12817480 |
| IF | 1.725 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 3.3 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pst15793 pst16212 |