| RRC ID |
86896
|
| Author |
Rikako Sanuki, Hatsune Minami, Emi Kawano, Keita Katsuma, Toshiyuki Takano-Shimizu-Kouno, Shosuke Yoshida
|
| Title |
Polyethylene terephthalate degradation by Drosophila melanogaster through heterologous expression of glycosylated polyethylene terephthalate hydrolase (PETase)
|
| Journal |
Communications Sustainability
|
| Abstract |
Polyethylene terephthalate (PET) is one of the most widely used plastics, yet recycling relies on energy-intensive chemical processes with environmental burdens. As an alternative, PET hydrolytic enzymes such as PET hydrolase (PETase) from
Piscinibacter sakaiensis
have been proposed for biological PET recycling under mild conditions. Inspired by recent studies on plastic degradation by insects, here we explored genetically engineered insects as a platform for PET biodegradation. We heterologously expressed PETase in the fruit fly
Drosophila melanogaster
, targeting a subset of the intestinal tract and the salivary glands. PETase-expressing flies degraded both a water-soluble PET copolymer
in vivo
and solid PET in the surrounding environment under alkaline conditions. Notably, the secreted PETase was glycosylated, reducing catalytic activity but enhancing stability and enabling prolonged PET degradation. These findings demonstrate a proof of concept for the application of engineered insects in plastic biodegradation and suggest new directions for sustainable, biology-based recycling technologies.
|
| Volume |
1
|
| Published |
2026-2-25
|
| DOI |
10.1038/s44458-026-00047-5
|
| Resource |
| Drosophila |
DGRC#119680
DGRC#119681
DGRC#119682
DGRC#119683
DGRC#112663
DGRC#108231
DGRC#130449 |
