論文 - 詳細
| RRC ID | 86912 |
|---|---|
| 著者 | Wessel EM, Drummond-Barbosa D. |
| タイトル | Glycolysis is intrinsically required in the follicle stem cell lineage but not in female germline stem cells or their early progeny in Drosophila melanogaster. |
| ジャーナル | Genetics |
| Abstract |
Multiple tissue stem cells depend on glycolysis or β-oxidation for cell fate decisions. However, how universal these requirements are and how they change as stem cell daughters undergo differentiation remains unclear. The Drosophila ovary is a powerful stem cell model with two distinct stem cell populations: germline stem cells (GSCs), which produce oocytes to perpetuate the species, and follicle stem cells (FSCs), a somatic lineage. Several studies have begun addressing the roles of metabolism within the Drosophila female GSC lineage, but direct systematic analyses of glycolysis and/or mitochondrial fatty acid β-oxidation requirements across these lineages have been lacking. Here, using genetic mosaic analysis with null alleles, we found that genes encoding key regulatory glycolytic enzymes-Phosphofructokinase (Pfk) and Pyruvate kinase (Pyk)-are not cell autonomously required for GSC maintenance, proliferation, or early differentiation through 16-cell germline cyst formation and oocyte specification. Although germline cysts lacking Pfk or Pyk function can develop through early vitellogenesis, they grow slowly and display impaired nurse cell chromatin dispersal. By contrast, FSCs and their early daughters require Pfk (but not Pyk) for normal survival, while later follicle cells need both Pfk and Pyk for survival and only Pfk for proliferation, suggesting that follicle cells predominantly require glycolytic intermediates upstream of Pyk. Surprisingly, mitochondrial β-oxidation was dispensable in both lineages. These findings uncover an unusual metabolic state in GSCs and their early daughters, with marked differences from the neighboring FSC lineage and other somatic stem cells. |
| 公開日 | 2026-1-20 |
| DOI | 10.1093/genetics/iyag015 |
| PII | 8431687 |
| PMID | 41557640 |
| PMC | PMC12928705 |
| MeSH | Animals Cell Differentiation Cell Lineage Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster* / cytology Drosophila melanogaster* / genetics Drosophila melanogaster* / metabolism Female Glycolysis* Ovarian Follicle* / cytology Ovarian Follicle* / metabolism Pyruvate Kinase / genetics Pyruvate Kinase / metabolism Stem Cells* / cytology Stem Cells* / metabolism |
| IF | 4.015 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 2.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#116261 DGRC#116262 |