論文 - 詳細
| RRC ID | 86926 |
|---|---|
| 著者 | Onuma M, Kumagai T, Hayashi K, Kamei Y, Takahashi A. |
| タイトル | Heat-mediated manipulation of gene expression by IR-LEGO in the developing genitalia in Drosophila. |
| ジャーナル | G3 (Bethesda) |
| Abstract |
Manipulating gene expression in a tissue-specific and temporally controlled manner is essential for understanding the function of the focal genes. Still, in many cases, the limited availability of specific promotors to drive ectopic manipulation remains a restricting factor in developing organs, even in Drosophila. Developing external genitalia is one such organ with a complex anatomical structure shaped by a joint regulatory network of many transcription factors. To overcome the restriction, we employed the infrared laser-evoked gene operator system (IR-LEGO), in which infrared laser (1,480 nm) irradiation induces gene expression under the control of a heat shock promoter. Pupal genital structures were irradiated at approximately 24 or 48 hours after puparium formation. We tested a range of laser power and depth to the target structure by a reporter assay using green fluorescent protein, which was induced under the control of the heat shock protein 70 promoter (hs-GAL4). In previous studies, the IR-LEGO has been used as a tool to induce ectopic transgene expression. In this study, we attempted to knock down genes such as yellow (y) and odd-paired (opa) ectopically by RNAi using the GAL4/UAS system. The results demonstrated that this technique has a high potential in manipulating transcript abundance levels in small groups of cells in specific genital structures to unravel novel functions of genes involved in the morphogenesis of species-specific and rapidly evolving anatomical structures. |
| 公開日 | 2026-2-12 |
| DOI | 10.1093/g3journal/jkag035 |
| PII | 8475299 |
| PMID | 41677373 |
| PMC | PMC13042308 |
| MeSH | Animals Drosophila* / genetics Drosophila* / growth & development Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster* / genetics Drosophila melanogaster* / growth & development Gene Expression Regulation, Developmental* / radiation effects Genitalia* / growth & development Genitalia* / metabolism Hot Temperature* Lasers Promoter Regions, Genetic RNA Interference Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 2.781 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 1.2 |
| リソース情報 | |
| ショウジョウバエ | 3757R-1 1133R-3 DGRC#106509 |