論文 - 詳細
| RRC ID | 86976 |
|---|---|
| 著者 | Yoshimi K, Yamauchi Y, Tanaka T, Shimada T, Sato M, Mashimo T. |
| タイトル | Photoactivatable Cre knock-in mice for spatiotemporal control of genetic engineering in vivo. |
| ジャーナル | Lab Invest |
| Abstract |
Although the Cre-loxP recombination system has been extensively used to analyze gene function in vivo, spatiotemporal control of Cre activity is a critical limitation for easy and precise recombination. Here, we established photoactivatable-Cre (PA-Cre) knock-in (KI) mice at a safe harbor locus for the spatial and temporal regulation of Cre recombinase activity. The mice showed whole-body Cre recombination activity following light exposure for only 1 h. Almost no leaks of Cre recombination activity were detected in the KI mice under natural light conditions. Spot irradiation could induce locus-specific recombination noninvasively, enabling us to compare phenotypes on the left and right sides in the same mouse. Furthermore, long-term irradiation using an implanted wireless LED substantially improved Cre recombination activity, especially in the brain. These results demonstrate that PA-Cre KI mice can facilitate the spatiotemporal control of genetic engineering and provide a useful resource to elucidate gene function in vivo with Cre-loxP. |
| 巻・号 | 101(1) |
| ページ | 125-135 |
| 公開日 | 2021-1-1 |
| DOI | 10.1038/s41374-020-00482-5 |
| PII | S0023-6837(22)00325-7 |
| PMID | 32892213 |
| MeSH | Animals Female Gene Knock-In Techniques* Genetic Engineering Green Fluorescent Proteins / genetics* Integrases / genetics* Luminescent Proteins / genetics* Mice Mice, Inbred C57BL Optogenetics / methods* RNA, Untranslated / genetics Red Fluorescent Protein |
| IF | 4.197 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 実験動物マウス | RBRC04874 |