| RRC ID |
86976
|
| 著者 |
Yoshimi K, Yamauchi Y, Tanaka T, Shimada T, Sato M, Mashimo T.
|
| タイトル |
Photoactivatable Cre knock-in mice for spatiotemporal control of genetic engineering in vivo.
|
| ジャーナル |
Lab Invest
|
| Abstract |
Although the Cre-loxP recombination system has been extensively used to analyze gene function in vivo, spatiotemporal control of Cre activity is a critical limitation for easy and precise recombination. Here, we established photoactivatable-Cre (PA-Cre) knock-in (KI) mice at a safe harbor locus for the spatial and temporal regulation of Cre recombinase activity. The mice showed whole-body Cre recombination activity following light exposure for only 1 h. Almost no leaks of Cre recombination activity were detected in the KI mice under natural light conditions. Spot irradiation could induce locus-specific recombination noninvasively, enabling us to compare phenotypes on the left and right sides in the same mouse. Furthermore, long-term irradiation using an implanted wireless LED substantially improved Cre recombination activity, especially in the brain. These results demonstrate that PA-Cre KI mice can facilitate the spatiotemporal control of genetic engineering and provide a useful resource to elucidate gene function in vivo with Cre-loxP.
|
| 巻・号 |
101(1)
|
| ページ |
125-135
|
| 公開日 |
2021-1-1
|
| DOI |
10.1038/s41374-020-00482-5
|
| PII |
S0023-6837(22)00325-7
|
| PMID |
32892213
|
| MeSH |
Animals
Female
Gene Knock-In Techniques*
Genetic Engineering
Green Fluorescent Proteins / genetics*
Integrases / genetics*
Luminescent Proteins / genetics*
Mice
Mice, Inbred C57BL
Optogenetics / methods*
RNA, Untranslated / genetics
Red Fluorescent Protein
|
| リソース情報 |
| 実験動物マウス |
RBRC04874 |
