論文 - 詳細
| RRC ID | 87016 |
|---|---|
| 著者 | Tossell K, Dodhia RA, Galet B, Tkachuk O, Ungless MA. |
| タイトル | Tonic GABAergic inhibition, via GABAA receptors containing αβƐ subunits, regulates excitability of ventral tegmental area dopamine neurons. |
| ジャーナル | Eur J Neurosci |
| Abstract |
The activity of midbrain dopamine neurons is strongly regulated by fast synaptic inhibitory γ-Aminobutyric acid (GABA)ergic inputs. There is growing evidence in other brain regions that low concentrations of ambient GABA can persistently activate certain subtypes of GABAA receptor to generate a tonic current. However, evidence for a tonic GABAergic current in midbrain dopamine neurons is limited. To address this, we conducted whole-cell recordings from ventral tegmental area (VTA) dopamine neurons in brain slices from mice. We found that application of GABAA receptor antagonists decreased the holding current, indicating the presence of a tonic GABAergic input. Global increases in GABA release, induced by either a nitric oxide donor or inhibition of GABA uptake, further increased this tonic current. Importantly, prolonged inhibition of the firing activity of local GABAergic neurons abolished the tonic current. A combination of pharmacology and immunohistochemistry experiments suggested that, unlike common examples of tonic inhibition, this current may be mediated by a relatively unusual combination of α4βƐ subunits. Lastly, we found that the tonic current reduced excitability in dopamine neurons suggesting a subtractive effect on firing activity. |
| 巻・号 | 53(6) |
| ページ | 1722-1737 |
| 公開日 | 2021-3-1 |
| DOI | 10.1111/ejn.15133 |
| PMID | 33522050 |
| PMC | PMC8651010 |
| MeSH | Animals Dopaminergic Neurons* GABAergic Neurons Mice Patch-Clamp Techniques Receptors, GABA-A Synaptic Transmission Ventral Tegmental Area* gamma-Aminobutyric Acid |
| IF | 3.115 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Peer-review(Publons) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC02095 |