論文 - 詳細
| RRC ID | 87062 |
|---|---|
| 著者 | Araoyinbo A, Salat-Canela C, Vještica A. |
| タイトル | Gamete fusion triggers cytosolic functions and P-body recruitment of the RNA-binding protein Mei2 to drive fission yeast zygotic development. |
| ジャーナル | Genes Dev |
| Abstract |
Compartmentalized regulation of RNAs is emerging as a key driver of developmental transitions, with RNA-binding proteins performing specialized functions in different subcellular compartments. The RNA-binding protein Mei2, which arrests mitotic proliferation and drives zygotic development in fission yeast, was shown to function in the nucleus to trigger meiotic divisions. Here, using compartment-restricted alleles, we report that Mei2 functions in the cytosol to arrest mitotic growth and initiate development. We found that Mei2 is a zygote-specific component of P-bodies that inhibits the translation of tethered mRNAs. Importantly, we show that P-bodies are necessary for Mei2-driven development. Phosphorylation of Mei2 by the inhibitory Pat1 kinase impedes P-body recruitment of both Mei2 and its target RNA. Finally, we establish that Mei2 recruitment to P-bodies and its cytosolic functions, including translational repression of tethered RNAs, depend on the RNA-binding domain of Mei2 that is dispensable for nuclear Mei2 roles. Collectively, our results dissect how distinct pools of an RNA-binding protein control developmental stages and implicate P-bodies as key regulators of gamete-to-zygote transition. |
| 巻・号 | 40(5-6) |
| ページ | 328-350 |
| 公開日 | 2026-3-2 |
| DOI | 10.1101/gad.353201.125 |
| PII | gad.353201.125 |
| PMID | 41298081 |
| PMC | PMC12951761 |
| MeSH | Cytosol* / metabolism Gene Expression Regulation, Fungal Phosphorylation RNA, Messenger / metabolism RNA-Binding Proteins* / genetics RNA-Binding Proteins* / metabolism Schizosaccharomyces* / cytology Schizosaccharomyces* / genetics Schizosaccharomyces* / growth & development Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins* / genetics Schizosaccharomyces pombe Proteins* / metabolism Zygote* / cytology Zygote* / growth & development Zygote* / metabolism |
| IF | 9.527 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.4 |
| リソース情報 | |
| 酵母 | |