論文 - 詳細
| RRC ID | 87212 |
|---|---|
| 著者 | Hiramatsu N, Yamamoto N, Ohkuma M, Nagai N, Miyachi EI, Yamatsuta K, Imaizumi K. |
| タイトル | Iris-derived induced pluripotent stem cells that express GFP in all somatic cells of mice and differentiate into functional retinal neurons. |
| ジャーナル | Med Mol Morphol |
| Abstract |
When regenerated tissue is generated from induced pluripotent stem cells (iPSCs), it is necessary to track and identify the transplanted cells. Fluorescently-labeled iPSCs synthesize a fluorescent substance that is easily tracked. However, the expressed protein should not affect the original genome sequence or pluripotency. To solve this problem, we created a cell tool for basic research on iPSCs. Iris tissue-derived cells from GFP fluorescence-expressing mice (GFP-DBA/2 mice) were reprogrammed to generate GFP mouse iris-derived iPSCs (M-iris GFP iPSCs). M-iris GFP iPSCs expressed cell markers characteristic of iPSCs and showed pluripotency in differentiating into the three germ layers. In addition, when expressing GFP, the cells differentiated into functional recoverin- and calbindin-positive cells. Thus, this cell line will facilitate future studies on iPSCs. |
| 巻・号 | 55(4) |
| ページ | 292-303 |
| 公開日 | 2022-12-1 |
| DOI | 10.1007/s00795-022-00330-z |
| PII | 10.1007/s00795-022-00330-z |
| PMID | 35932315 |
| MeSH | Animals Calbindins / metabolism Cell Differentiation Induced Pluripotent Stem Cells* / metabolism Iris* / cytology Mice Mice, Inbred DBA Recoverin / metabolism Retinal Neurons* / metabolism |
| IF | 2.429 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00267 |