| RRC ID |
87212
|
| Author |
Hiramatsu N, Yamamoto N, Ohkuma M, Nagai N, Miyachi EI, Yamatsuta K, Imaizumi K.
|
| Title |
Iris-derived induced pluripotent stem cells that express GFP in all somatic cells of mice and differentiate into functional retinal neurons.
|
| Journal |
Med Mol Morphol
|
| Abstract |
When regenerated tissue is generated from induced pluripotent stem cells (iPSCs), it is necessary to track and identify the transplanted cells. Fluorescently-labeled iPSCs synthesize a fluorescent substance that is easily tracked. However, the expressed protein should not affect the original genome sequence or pluripotency. To solve this problem, we created a cell tool for basic research on iPSCs. Iris tissue-derived cells from GFP fluorescence-expressing mice (GFP-DBA/2 mice) were reprogrammed to generate GFP mouse iris-derived iPSCs (M-iris GFP iPSCs). M-iris GFP iPSCs expressed cell markers characteristic of iPSCs and showed pluripotency in differentiating into the three germ layers. In addition, when expressing GFP, the cells differentiated into functional recoverin- and calbindin-positive cells. Thus, this cell line will facilitate future studies on iPSCs.
|
| Volume |
55(4)
|
| Pages |
292-303
|
| Published |
2022-12-1
|
| DOI |
10.1007/s00795-022-00330-z
|
| PII |
10.1007/s00795-022-00330-z
|
| PMID |
35932315
|
| MeSH |
Animals
Calbindins / metabolism
Cell Differentiation
Induced Pluripotent Stem Cells* / metabolism
Iris* / cytology
Mice
Mice, Inbred DBA
Recoverin / metabolism
Retinal Neurons* / metabolism
|
| Resource |
| Mice |
RBRC00267 |
