| RRC ID |
87283
|
| Author |
Krunic A, Bellio TA, Cohen BZ, Labadorf A, Stein TD, Lin H, Mellott TJ, Blusztajn JK.
|
| Title |
Brain DNA Methylation Atlas of AppNL-G-F Alzheimer's Disease Model Mice Across Age and Region Reveals Choline-Induced Resilience.
|
| Journal |
Aging Cell
|
| Abstract |
Alzheimer's disease (AD) is the most common type of dementia. Current treatments for AD are inadequate, and there is a need to design preventive strategies that would improve the resistance or resilience to AD pathology. Because aberrant brain DNA methylation (DNAm) is associated with hallmarks of AD, we tested the hypothesis that a nutritional approach using choline, an essential nutrient and methyl donor, would modulate DNAm to ameliorate AD pathologies. Previous studies showed that perinatal choline supplementation (PCS) reduced AD-like neuropathology and inflammation while improving cognitive performance in AD mouse models. Here we investigated hippocampal and cerebral cortical DNAm patterns by reduced representation bisulfite sequencing from 3 to 12 months in wild-type (WT) and AppNL-G-F AD model mice fed a 1.1 g/kg control or 5.5 g/kg PCS diet from conception to weaning. AppNL-G-F mice showed extensive CpG DNAm changes, which were associated with the age-dependent progression of amyloidosis. PCS induced genotype-specific DNAm patterns and reversed DNAm changes in multiple genes in AppNL-G-F mice. By associating DNAm with matched transcriptomics, we found that DNAm in AppNL-G-F mice correlated with the expression of microglial genes, while DNAm-associated genes modulated by PCS were related to synaptic function. Moreover, we found that methylation levels of several CpGs were associated with levels of beta amyloidosis, relating epigenetic changes to neuropathology. Overall, our data suggest that DNAm in the brain serves as an epigenetic mechanism for abnormal gene expression in AppNL-G-F mice and indicate that PCS may promote resilience to synaptic dysfunction through modulating DNAm.
|
| Volume |
24(11)
|
| Pages |
e70241
|
| Published |
2025-11-1
|
| DOI |
10.1111/acel.70241
|
| PMID |
41081550
|
| PMC |
PMC12611327
|
| MeSH |
Aging* / genetics
Alzheimer Disease* / genetics
Alzheimer Disease* / metabolism
Alzheimer Disease* / pathology
Animals
Brain* / drug effects
Brain* / metabolism
Brain* / pathology
Choline* / pharmacology
DNA Methylation* / drug effects
DNA Methylation* / genetics
Disease Models, Animal
Female
Male
Mice
Mice, Transgenic
|
| Resource |
| Mice |
RBRC06344 |
