Reference - Detail
| RRC ID | 87312 |
|---|---|
| Author | Nagata W, Kodama K, Nakagawa K, Ishizuka T. |
| Title | Lysophosphatidic acid regulates macrophage polarization via LPAR1 by suppressing inflammatory responses and promoting M2-like characteristics. |
| Journal | Clin Exp Immunol |
| Abstract |
Lysophosphatidic acid (LPA) is a crucial bioactive lipid mediator involved in various physiological processes; however, its role in macrophage polarization remains poorly understood; therefore, this study aimed to elucidate the modulatory effect of LPA on macrophage polarization, particularly its ability to shift M1 macrophages towards an M2-like phenotype, using murine macrophage RAW264.7 cells to confirm the expression of LPA receptor 1 (LPAR1) through immunofluorescence staining, which revealed that treatment of resting MO macrophages with LPA decreased inflammatory cytokines (IL-6, TNF-α) and increased TGF-β, with similar effects observed in LPS-stimulated cells and reversed by the LPAR1 inhibitor AM095, and immunostaining demonstrated a notable shift from an M1- to M2-like phenotype, as evidenced by an increase in the arginase-1/CD68 ratio; furthermore, LPA significantly decreased lactate production and increased ATP production in M1 macrophages, promoting a shift towards oxidative phosphorylation and suggesting metabolic reprogramming towards an M2-like phenotype, significantly influencing macrophage polarization and promoting a shift from a pro-inflammatory M1-like phenotype to an anti-inflammatory M2-like phenotype; these results suggest that treatment with LPA may help ameliorate diseases characterized by aberrant macrophage polarization, providing insights for the development of potential therapeutic strategies for inflammatory and autoimmune diseases. |
| Volume | 220(1) |
| Published | 2026-1-6 |
| DOI | 10.1093/cei/uxaf083 |
| PII | 8385282 |
| PMID | 41423802 |
| PMC | PMC12782111 |
| MeSH | Animals Cell Polarity / drug effects Cytokines / metabolism Inflammation* / immunology Inflammation* / metabolism Lipopolysaccharides / pharmacology Lysophospholipids* / pharmacology Macrophage Activation / drug effects Macrophages* / drug effects Macrophages* / immunology Macrophages* / metabolism Mice RAW 264.7 Cells Receptors, Lysophosphatidic Acid* / metabolism |
| IF | 3.532 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 1.7 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB053) |