| RRC ID |
87406
|
| Author |
Yoshioka I, Fahal AH, Sow D, Kaneko S, Mori Y, Ban S, Yaguchi T.
|
| Title |
Simultaneous Detection of Four Madurella Species Using Loop-Mediated Isothermal Amplification (LAMP) for Eumycetoma Diagnosis.
|
| Journal |
Mycopathologia
|
| Abstract |
Eumycetoma is a neglected tropical disease caused primarily by a Madurella mycetomatis infection, besides other related species. In this study, we designed a novel loop-mediated isothermal amplification (LAMP) primer set capable of simultaneously detecting four Madurella species (M. mycetomatis, M. pseudomycetomatis, M. tropicana, and M. fahalii). Genomic sequencing of M. pseudomycetomatis strain and comparative genome analysis revealed the candidate genes that were common among and specific to Madurella species. The 3 LAMP primer sets targeting these genes detected up to 1 pg of the genomic DNA of all 4 Madurella species, exhibiting no cross-reactivity toward other pathogenic fungi. Among these, one primer set showing better reactivity was selected as a candidate used for diagnosis. Therefore, we developed novel primer sets which enabled the simultaneous detection of four Madurella species. Our present findings will lead to a faster and simpler diagnostic tool for eumycetoma detection, especially in rural clinical settings.
|
| Volume |
190(6)
|
| Pages |
105
|
| Published |
2025-10-29
|
| DOI |
10.1007/s11046-025-01019-4
|
| PII |
10.1007/s11046-025-01019-4
|
| PMID |
41152542
|
| PMC |
PMC12568910
|
| MeSH |
DNA Primers / genetics
DNA, Fungal / genetics
Humans
Madurella* / classification
Madurella* / genetics
Madurella* / isolation & purification
Molecular Diagnostic Techniques* / methods
Mycetoma* / diagnosis
Mycetoma* / microbiology
Nucleic Acid Amplification Techniques* / methods
Sensitivity and Specificity
|
| IF |
2.452
|
| Resource |
| Pathogenic eukaryotic microorganisms |
Madurella mycetomatis IFM 46458, IFM 68169, IFM 68172, IFM 68173, IFM 68174, IFM 68175 etc, Madurella pseudomycetomatis IFM 46460, Madurella fahalii IFM 68170, IFM 68171, IFM 68242 |
| General Microbes |
JCM7436
JCM3388 |
