論文 - 詳細
| RRC ID | 87424 |
|---|---|
| 著者 | Xu J, Hori T, Okeyo KO, Tsurumi B, Yoshida S, Nashimoto Y, Kaji H. |
| タイトル | Microstructured chitosan mesh scaffold for efficient production of cell-cultured meat. |
| ジャーナル | Sci Rep |
| Abstract |
The rising global demand for sustainable food solutions has been a key driver of significant advancements in cell-cultured meat technology. However, challenges remain in efficiently inducing muscle cell maturation and constructing layered structures that closely resemble natural meat. In this study, we developed a novel microstructured chitosan mesh scaffold to address these challenges, with a focus on promoting cell alignment and minimizing non-cellular components in the final product. Chitosan, a biocompatible and edible polymer derived from chitin, was processed into customizable micromesh sheets to support three-dimensional cell culture. Incorporating gelatin into the chitosan matrix significantly enhanced the adhesion of C2C12 mouse myoblasts to the mesh sheets. Cell culture experiments demonstrated that the chitosan-gelatin mesh scaffold supports robust cell proliferation, leading to the formation of uniform cell sheets. Notably, the rhombus-shaped mesh openings effectively promoted cell alignment, a critical factor for inducing muscle cell maturation and replicating the anisotropic structure of natural muscle fibers. This approach has the potential to not only reduce the scaffolding material-to-cell ratio but also facilitate the creation of muscle fibers with enhanced structural and textural properties. |
| 公開日 | 2026-4-5 |
| DOI | 10.1038/s41598-026-46287-2 |
| PII | 10.1038/s41598-026-46287-2 |
| PMID | 41936631 |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.7 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |