| RRC ID |
87497
|
| Author |
Kim J, Hwang Y, Kim S, Kwon D, Park J, Cho B, An S, Kang S, Kim Y, Kim S, Lengner CJ, Kim S, Kwon Y, Sung JS, Kim J.
|
| Title |
Electromagnetic field-inducible in vivo gene switch for remote spatiotemporal control of gene expression.
|
| Journal |
Cell
|
| Abstract |
Gaining precise control of gene expression is crucial in biomedical applications. However, spatiotemporal precision remains challenging. Here, we present a remotely controlled in vivo gene switch responsive to electromagnetic fields (EMFs) that enables precise spatiotemporal activation of target genes. We uncovered the EMF-inducible gene switch activation mechanism via a CRISPR-Cas9 screen, identifying cytochrome b5 type B (Cyb5b) as an essential mediator likely acting as an EMF sensor. The EMF-inducible gene switch was activated by rhythmic oscillatory calcium dynamics rather than generic calcium influx, defining a precisely tuned and bio-orthogonal induction mechanism. Functionally, EMF activation of the Oct4-Sox2-Klf4 (OSK) cassette induced in vivo partial reprogramming in aged mice, conditional expression of human mutant amyloid precursor protein (APP) for Alzheimer's disease (AD) modeling recapitulated pathological features, and EMF-mediated Tph2 expression restored serotonergic activity and ameliorated depressive-like behaviors in Tph2-mutant depression mice. Overall, a remotely controlled EMF-inducible gene switch represents a versatile and effective biomedical platform.
|
| Published |
2026-4-14
|
| DOI |
10.1016/j.cell.2026.03.029
|
| PII |
S0092-8674(26)00330-2
|
| PMID |
41985457
|
| Resource |
| Mice |
RBRC06344 |
