論文 - 詳細
| RRC ID | 87511 |
|---|---|
| 著者 | Kanai SS, Fujioka H, Kawatani M, Spratt SJ, Fukuta SS, Zhan Z, Ma Y, Kuruma K, Asanuma D, Asada Y, Taki M, Yamaguchi S, Ozeki Y, Kamiya M. |
| タイトル | Diversification of the 10th core atom of 9-cyanopyronins expands the resonance Raman vibrational palette. |
| ジャーナル | Commun Chem |
| Abstract |
9-Cyanopyronin derivatives have been used as resonance Raman probes for highly sensitive and multiplexed vibrational biological imaging. Here, we synthesized a series of fourteen 9-cyanopyronin derivatives in which the 10th core atom is substituted with various group 13, 14, 15 or 16 elements, and systematically evaluated their properties using electronic pre-resonance stimulated Raman scattering (EPR-SRS) microscopy. Substitution at the 10th position modulates not only the vibrational frequency of the nitrile (C ≡ N) group in the silent region, but also the conjugated double-bond stretching frequency (C = C) in the fingerprint region, and additionally alters the subcellular localization of the probes. By employing different combinations of probes, we were able to utilize the distinct patterns of C ≡ N or C = C bond vibrations together with the different subcellular localizations of the probes to achieve live-cell three-color imaging. These 9-cyanopyronin derivatives greatly expand the vibrational palette available for multiplexed SRS imaging. |
| 公開日 | 2026-4-16 |
| DOI | 10.1038/s42004-026-02019-1 |
| PII | 10.1038/s42004-026-02019-1 |
| PMID | 41992045 |
| IF | 4.253 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 4.7 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |