| RRC ID |
87511
|
| 著者 |
Kanai SS, Fujioka H, Kawatani M, Spratt SJ, Fukuta SS, Zhan Z, Ma Y, Kuruma K, Asanuma D, Asada Y, Taki M, Yamaguchi S, Ozeki Y, Kamiya M.
|
| タイトル |
Diversification of the 10th core atom of 9-cyanopyronins expands the resonance Raman vibrational palette.
|
| ジャーナル |
Commun Chem
|
| Abstract |
9-Cyanopyronin derivatives have been used as resonance Raman probes for highly sensitive and multiplexed vibrational biological imaging. Here, we synthesized a series of fourteen 9-cyanopyronin derivatives in which the 10th core atom is substituted with various group 13, 14, 15 or 16 elements, and systematically evaluated their properties using electronic pre-resonance stimulated Raman scattering (EPR-SRS) microscopy. Substitution at the 10th position modulates not only the vibrational frequency of the nitrile (C ≡ N) group in the silent region, but also the conjugated double-bond stretching frequency (C = C) in the fingerprint region, and additionally alters the subcellular localization of the probes. By employing different combinations of probes, we were able to utilize the distinct patterns of C ≡ N or C = C bond vibrations together with the different subcellular localizations of the probes to achieve live-cell three-color imaging. These 9-cyanopyronin derivatives greatly expand the vibrational palette available for multiplexed SRS imaging.
|
| 公開日 |
2026-4-16
|
| DOI |
10.1038/s42004-026-02019-1
|
| PII |
10.1038/s42004-026-02019-1
|
| PMID |
41992045
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007) |
