| RRC ID |
87515
|
| 著者 |
de Cubas L, Crevatin MF, Vega M, Boronat S, Ayté J, Hidalgo E.
|
| タイトル |
Dose-dependent mitochondrial H2O2 signaling drives toxicity or stress adaptation and longevity in fission yeast.
|
| ジャーナル |
Cell Rep
|
| Abstract |
The spatiotemporal dynamics of hydrogen peroxide (H2O2) signaling and its effects on gene expression and cell fitness remain unclear. Using fission yeast, we applied genetic tools to control and monitor intracellular H2O2 levels. We expressed the H2O2 biosensor HyPer7 in four subcellular compartments, overexpressed D-amino acid oxidase (Dao1) in specific locations to induce localized H2O2 production, and modulated H2O2 detoxification or sensing. H2O2 concentrations showed 2- to 5-fold reductions across membranes, and D-amino acid treatments generated nanomolar H2O2 levels in Dao1-expressing cells. Mitochondrial-targeted Dao1 produced H2O2 fluxes capable of reaching the nucleus. While high mitochondrial H2O2 disrupted mitochondrial morphology and respiration, lower levels triggered antioxidant defenses, enhancing stress resistance and longevity. These findings establish a quantitative framework for mitochondrial H2O2 signaling and demonstrate that localized redox signals from mitochondria can either promote or impair cellular fitness depending on their intensity.
|
| 巻・号 |
45(4)
|
| ページ |
117277
|
| 公開日 |
2026-4-16
|
| DOI |
10.1016/j.celrep.2026.117277
|
| PII |
S2211-1247(26)00355-4
|
| PMID |
41996234
|
| リソース情報 |
| 酵母 |
FY16151 |
