論文 - 詳細
| RRC ID | 87562 |
|---|---|
| 著者 | Zhu X, Smith MVE, Tanentzapf G. |
| タイトル | Automated detection of spontaneous calcium signaling events in prohemocytes of the Drosophila melanogaster lymph gland. |
| ジャーナル | MicroPubl Biol |
| Abstract |
Calcium signaling is an important regulator of stem cell maintenance and differentiation. Here we report the development of an image processing pipeline for ex vivo time-lapse microscopy data that enables the unbiased, automated detection of calcium signaling events in prohemocytes of the Drosophila melanogaster lymph gland. We also show that heterogeneity in gene expression driven by Tep4-Gal4, which is used to mark prohemocytes, accounts for most of the cell-to-cell variability in the signal, and that spontaneous calcium signaling events in the lymph gland can last from a few seconds to well over a minute. |
| 巻・号 | 2025 |
| 公開日 | 2025-1-1 |
| DOI | 10.17912/micropub.biology.001883 |
| PMID | 41328327 |
| PMC | PMC12665145 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#105442 |