論文 - 詳細
| RRC ID | 87578 |
|---|---|
| 著者 | Girard V, Sorge S, Kurth J, Alexandre C, Gould AP. |
| タイトル | ShineGAL4 drivers for tissue and cell-type specific optogenetics in Drosophila. |
| ジャーナル | Development |
| Abstract |
An optogenetic split-GAL4 system, ShineGAL4, allows genes to be manipulated with unprecedented spatiotemporal precision. Here, we convert a panel of 14 GAL4 drivers widely used in Drosophila research into their ShineGAL4 counterparts. Homology assisted CRISPR knock-in (HACK) is used to replace GAL4 with the GAL4 DNA binding domain fused to a Magnet photoswitch. We show that the resulting ShineGAL4 drivers enable gene expression to be rapidly induced by light specifically in fat body, muscles, enterocytes, oenocytes, Malpighian tubules, neurons, neuroblast lineages, glial subtypes or in all glia. We also develop an optogenetic cassette for photoactivation of GAL4 in 'silent' FLP-out clones. This panel of optogenetic tools will enable precise spatiotemporal control of gene expression in a wide range of different Drosophila tissues and cell-types. |
| 巻・号 | 153(4) |
| 公開日 | 2026-2-15 |
| DOI | 10.1242/dev.204981 |
| PII | 370848 |
| PMID | 41738557 |
| PMC | PMC12967137 |
| MeSH | Animals CRISPR-Cas Systems / genetics Drosophila* / genetics Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster* / genetics Neurons / metabolism Optogenetics* / methods Organ Specificity / genetics Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 5.611 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 30 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 17.6 |
| リソース情報 | |
| ショウジョウバエ | DGRC#112622 |