論文 - 詳細
| RRC ID | 87580 |
|---|---|
| 著者 | Zhang Y, Feng Y, Ye X, Lin Y, Zeng L, Luo Y, Zhou L, Shen C, Hu W, Yan D, Lin X. |
| タイトル | Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production. |
| ジャーナル | Development |
| Abstract |
Precise control of morphogen dosage in the source is essential for establishing developmental patterning and maintaining signaling stability. Here, we demonstrate that in Drosophila wing imaginal discs Decapentaplegic and BMP (Dpp/BMP) signaling modulates expression of the ligand Dpp through direct transcriptional auto-regulation. The results show that reduction of Dpp/BMP signaling upregulates dpp transcription, whereas enhanced signaling represses it. This transcriptional negative-feedback mechanism primarily depends on the BMP-silencer-element (BMP-SE) complex binding to BMP-SE motifs at the dpp downstream distal cis-regulatory region. We validated this mechanism through BMP-SE complex interference and BMP-SE motif disruption assays. Our findings extend the morphogen source-sink theory by uncovering that the Dpp/BMP signaling pathway dynamically balances Dpp dosage via transcriptional auto-modulation, thereby ensuring optimal ligand production for signaling homeostasis. |
| 巻・号 | 152(21) |
| 公開日 | 2025-11-1 |
| DOI | 10.1242/dev.204488 |
| PII | 369186 |
| PMID | 40955849 |
| PMC | PMC12633792 |
| MeSH | Animals Bone Morphogenetic Proteins* / genetics Bone Morphogenetic Proteins* / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster* / genetics Drosophila melanogaster* / metabolism Gene Expression Regulation, Developmental Imaginal Discs / metabolism Ligands Signal Transduction* / genetics Transcription, Genetic* Wings, Animal / metabolism |
| IF | 5.611 |
| リソース情報 | |
| ショウジョウバエ | DGRC#106645 DGRC#108956 |