論文 - 詳細
| RRC ID | 87661 |
|---|---|
| 著者 | Narbonne-Reveau K, Erni A, Eichner N, Sankar S, Kapoor S, Meister G, Cremer H, Maurange C, Beclin C. |
| タイトル | In vivo AGO-APP identifies a module of microRNAs cooperatively preserving neural progenitors. |
| ジャーナル | PLoS Genet |
| Abstract |
MicroRNAs are essential regulators of gene expression. Their function is particularly important during neurogenesis, when the production of large numbers of neurons from a limited number of neural stem cells depends on the precise control of determination, proliferation and differentiation. However, microRNAs can target many mRNAs and vice-versa, raising the question of how specificity is achieved to elicit a precise regulatory response. Here we introduce in vivo AGO-APP, a novel approach to purify Argonaute-bound, and therefore active microRNAs from specific cell types. Using AGO-APP in the larval Drosophila central nervous system, we identify a module of microRNAs predicted to redundantly target all iconic genes known to control the transition from neuroblasts to neurons. While microRNA overexpression generally validated predictions, knockdown of individual microRNAs did not induce detectable phenotypes. In contrast, neuroblasts were induced to differentiate precociously when several microRNAs were knocked down simultaneously. Our data supports the concept that at physiological expression levels, the cooperative action of miRNAs allows efficient targeting of entire gene networks. |
| 巻・号 | 21(4) |
| ページ | e1011680 |
| 公開日 | 2025-4-1 |
| DOI | 10.1371/journal.pgen.1011680 |
| PII | PGENETICS-D-24-00423 |
| PMID | 40299997 |
| PMC | PMC12064045 |
| MeSH | Animals Argonaute Proteins* / genetics Argonaute Proteins* / metabolism Cell Differentiation / genetics Central Nervous System / growth & development Central Nervous System / metabolism Drosophila / genetics Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Gene Expression Regulation, Developmental Gene Regulatory Networks Larva / genetics MicroRNAs* / genetics MicroRNAs* / metabolism Neural Stem Cells* / cytology Neural Stem Cells* / metabolism Neurogenesis* / genetics Neurons / cytology Neurons / metabolism |
| IF | 5.175 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 23 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#112622 |