Reference - Detail
| RRC ID | 87826 |
|---|---|
| Author | Takahashi Y, Zhang Z, Tanaka I, Lee IT, He J, Koura Y, Sato S, Kojima H, Okabe T, Kiyono H, Sasaki T, Yamauchi Y, Kurashima Y, Sato R. |
| Title | Organoid phenotypic screening identified glycyrrhizin that confers protection against tumor necrosis factor-induced cell death. |
| Journal | Stem Cell Reports |
| Abstract |
Human organoids are considered physiological models that reflect human physiology; however, their applications in drug screening studies are limited. To develop a fundamental treatment for recurrent Crohn's disease (CD), in which tumor necrosis factor (TNF) is a key pathogenic factor, we conducted phenotypic drug screening to prevent TNF-induced cell death in human intestinal epithelial organoids. Glycyrrhizin, a natural product of licorice root, dose-dependently blocked TNF-induced cell death in organoids but not in TNF-sensitive L929 cells; L929 cells exhibited necroptosis, whereas organoid-derived cells preferentially showed apoptosis upon TNF treatment, determining the specificity of glycyrrhizin. Glycyrrhizin inhibited downstream caspase-8 signaling, which is essential for TNF-dependent apoptosis, and ameliorated intestinal inflammation in vivo. These results demonstrate that glycyrrhizin may be a novel therapeutic compound for CD and highlight the importance of using organoids for phenotypic drug screening. |
| Pages | 102891 |
| Published | 2026-4-16 |
| DOI | 10.1016/j.stemcr.2026.102891 |
| PII | S2213-6711(26)00102-5 |
| PMID | 41997150 |
| IF | 6.032 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 38 |
| Altmetric score changes over past 6months | 109.8 |
| Resource | |
| DNA material | CSII-EF-MCS-IRES2-Venus (RDB04384) |