論文 - 詳細
| RRC ID | 87847 |
|---|---|
| 著者 | Biswas KB, Nakagawa S, Sakamoto K. |
| タイトル | Epigenetic regulation of hyaluronan degradation and melanogenesis by Kawarayomogin I and II from Artemisia capillaris via miR-486-5p and miR-141-3p. |
| ジャーナル | Mol Biol Rep |
| Abstract |
BACKGROUND:We previously demonstrated that Artemisia capillaris flower extract (ACFE) suppresses the expression of HYBID (hyaluronan-binding protein involved in hyaluronan depolymerization), a hyaluronan-degrading enzyme, and increases the expression of miR-486-5p, a microRNA that downregulates HYBID. In the same study, we isolated two novel active compounds, Kawarayomogin I and II, and reported that they also suppress HYBID expression. However, it remained unclear whether their inhibitory effects on HYBID were mediated through miR-486-5p. Furthermore, because ACFE has been reported to inhibit melanin production, and miR-141-3p and miR-200a-3p are known to regulate melanogenesis, we investigated whether these compounds modulate melanogenesis-related microRNAs. METHODS AND RESULTS:The expressions levels of miR-486-5p in normal human dermal fibroblasts (NHDF) and miR-141-3p and miR-200a-3p in mouse B16 melanocytes were measured by RT-PCR. Melanin content was quantified by lysing the cells using NaOH followed by taking of absorbance at 420 nm. We confirmed for the first time that Kawarayomogin I and II significantly increased the expression of miR-486-5p in NHDF. Among miR-141-3p and miR-200a-3p, ACFE and Kawarayomogin I and II selectively induced miR-141-3p in B16. These findings identify Kawarayomogin I and II as the principal active components responsible for the melanin-suppressing activity of ACFE for the first time. Although these compounds may influence other microRNAs, our results suggest that they selectively regulate miR-486-5p and miR-141-3p, indicating an epigenetic mechanism underlying their biological effects. CONCLUSIONS:Collectively, Kawarayomogin I and II represent promising bioactive molecules that selectively and epigenetically regulate microRNA expression to confer dual functions in hyaluronan protection and melanogenesis suppression. |
| 巻・号 | 53(1) |
| 公開日 | 2026-5-2 |
| DOI | 10.1007/s11033-026-11887-8 |
| PII | 10.1007/s11033-026-11887-8 |
| PMID | 42068437 |
| MeSH | Animals Artemisia* / chemistry Artemisia* / metabolism Epigenesis, Genetic* / drug effects Fibroblasts / drug effects Fibroblasts / metabolism Humans Hyaluronic Acid* / metabolism Melanins* / biosynthesis Melanins* / metabolism Melanocytes / drug effects Melanocytes / metabolism Melanogenesis Mice MicroRNAs* / genetics MicroRNAs* / metabolism Plant Extracts / pharmacology |
| リソース情報 | |
| ヒト・動物細胞 | B16 melanoma(RCB1283) |