| RRC ID |
87854
|
| Author |
Yamaya A, Sonoda K, Kawakita T, Koshiishi Y, Shingai M, Matsumoto M, Seya T.
|
| Title |
ARNAX, but not conventional adjuvants, alum and squalene, induces antigen-specific CD8+ T cell proliferation in vaccination with influenza split vaccine.
|
| Journal |
Int Immunopharmacol
|
| Abstract |
The influenza split vaccine (ISV) is generally administered without adjuvant via intra-muscular (i.m.) or intranasal route. Research has demonstrated that SV alone exhibits no capacity to elicit a Th1 (T helper-1) response and cellular immunity represented by cytotoxic T lymphocytes (CTL) induction. This finding is consistent with the observation that SV merely up-regulates antibody (Ab) production in the pre-disposed population. This tendency is reproduced in the SV model in conjunction with conventional adjuvants, including alum or squalene. These adjuvants amplify the Th2 shift, consequently stimulating Th2-dependent Ab production in mice. Conventional adjuvants, when employed with SV in animal studies, demonstrate a limited capacity to stimulate cellular immune activation. The present study aims to demonstrate the efficacy of a Th1-inducing adjuvant, ARNAX, in promoting antigen-presenting DC and subsequent Ag-specific CTL proliferation, culminating in Th1 polarization. Consequently, the synthesis of the two effectors, CTL and Ab, is augmented by the incorporation of ARNAX in SV. The enhanced SV-mediated protective immunity may be further augmented by the induction of antigen-specific CTL and the unique profile of Th1-mediated Ab production.
|
| Volume |
159
|
| Pages |
114895
|
| Published |
2025-6-26
|
| DOI |
10.1016/j.intimp.2025.114895
|
| PII |
S1567-5769(25)00885-9
|
| PMID |
40409101
|
| MeSH |
Adjuvants, Immunologic* / pharmacology
Alum Compounds / pharmacology
Animals
Antibodies, Viral / blood
CD8-Positive T-Lymphocytes* / drug effects
CD8-Positive T-Lymphocytes* / immunology
Cell Proliferation / drug effects
Dendritic Cells / drug effects
Dendritic Cells / immunology
Female
Humans
Influenza Vaccines* / administration & dosage
Influenza Vaccines* / immunology
Lymphocyte Activation / drug effects
Mice
Mice, Inbred BALB C
Orthomyxoviridae Infections* / immunology
Orthomyxoviridae Infections* / prevention & control
Squalene / pharmacology
T-Lymphocytes, Cytotoxic / immunology
Th1 Cells / immunology
Vaccination
|
| Resource |
| Aged mice |
|
