Reference - Detail
| RRC ID | 87870 |
|---|---|
| Author | Seki T, Miyanari K, Shiraishi A, Tsuda S, Ansai S, Takeuchi H. |
| Title | Establishment of a transgenic strain for the whole brain calcium imaging in larval medaka fish (Oryzias latipes). |
| Journal | Neurosci Res |
| Abstract |
GCaMP-based calcium imaging is a powerful tool for investigating neural function in specific neurons. We generated transgenic (Tg) medaka strains expressing jGCaMP7s across extensive brain regions under the control of the gap43 promoter. Using these Tg larvae, calcium imaging successfully detected a tricaine-induced suppression of spontaneous neural activity and topographical visual responses in the optic tectum elicited by moving paramecia or optical fiber stimulation. These results indicate that our Tg medaka strains provide a versatile platform for investigating neural dynamics and their responses to various stimuli. |
| Volume | 219 |
| Pages | 104944 |
| Published | 2025-10-1 |
| DOI | 10.1016/j.neures.2025.104944 |
| PII | S0168-0102(25)00127-0 |
| PMID | 40769387 |
| MeSH | Animals Animals, Genetically Modified Brain* / metabolism Calcium* / metabolism Larva Neurons / metabolism Neurons / physiology Oryzias* / genetics Superior Colliculi / physiology |
| IF | 2.645 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 13 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Medaka | hi-medaka (MT835) |