| RRC ID |
87888
|
| 著者 |
Shimizu M, Hirabuchi A, Sugihara Y, Abe A, Takeda T, Kobayashi M, Hiraka Y, Kanzaki E, Oikawa K, Saitoh H, Langner T, Banfield MJ, Kamoun S, Terauchi R.
|
| タイトル |
A genetically linked pair of NLR immune receptors shows contrasting patterns of evolution.
|
| ジャーナル |
Proc Natl Acad Sci U S A
|
| Abstract |
Throughout their evolution, plant nucleotide-binding leucine-rich-repeat receptors (NLRs) have acquired widely divergent unconventional integrated domains that enhance their ability to detect pathogen effectors. However, the functional dynamics that drive the evolution of NLRs with integrated domains (NLR-IDs) remain poorly understood. Here, we reconstructed the evolutionary history of an NLR locus prone to unconventional domain integration and experimentally tested hypotheses about the evolution of NLR-IDs. We show that the rice (Oryza sativa) NLR Pias recognizes the effector AVR-Pias of the blast fungal pathogen Magnaporthe oryzae. Pias consists of a functionally specialized NLR pair, the helper Pias-1 and the sensor Pias-2, that is allelic to the previously characterized Pia pair of NLRs: the helper RGA4 and the sensor RGA5. Remarkably, Pias-2 carries a C-terminal DUF761 domain at a similar position to the heavy metal-associated (HMA) domain of RGA5. Phylogenomic analysis showed that Pias-2/RGA5 sensor NLRs have undergone recurrent genomic recombination within the genus Oryza, resulting in up to six sequence-divergent domain integrations. Allelic NLRs with divergent functions have been maintained transspecies in different Oryza lineages to detect sequence-divergent pathogen effectors. By contrast, Pias-1 has retained its NLR helper activity throughout evolution and is capable of functioning together with the divergent sensor-NLR RGA5 to respond to AVR-Pia. These results suggest that opposite selective forces have driven the evolution of paired NLRs: highly dynamic domain integration events maintained by balancing selection for sensor NLRs, in sharp contrast to purifying selection and functional conservation of immune signaling for helper NLRs.
|
| 巻・号 |
119(27)
|
| ページ |
e2116896119
|
| 公開日 |
2022-7-5
|
| DOI |
10.1073/pnas.2116896119
|
| PMID |
35771942
|
| PMC |
PMC9271155
|
| MeSH |
Evolution, Molecular*
Genetic Linkage
Host-Pathogen Interactions / immunology
Magnaporthe* / genetics
Magnaporthe* / pathogenicity
NLR Proteins* / genetics
NLR Proteins* / immunology
Oryza* / immunology
Oryza* / microbiology
Plant Diseases* / immunology
Plant Diseases* / microbiology
Plant Proteins* / genetics
Plant Proteins* / immunology
Protein Inhibitors of Activated STAT / genetics
Protein Inhibitors of Activated STAT / immunology
Receptors, Immunologic* / genetics
Receptors, Immunologic* / immunology
|
| IF |
9.412
|
| リソース情報 |
| イネ |
W1687
W0698
W1638
W2184
W0170
W2112
W1297
W1943
W1410
W1582
W0008
W0002
W0655
W0067(B) |
