| 著者 |
Zhang Y, Xiao G, Ding H, Zou Q, Gu D, Wen J, Pei Y, Guo R, Wang Q, Zhou X.
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| Abstract |
Two-component system (TCS) histidine kinases enable bacterial pathogens to sense environmental signals and regulate adaptive responses during infection. The EnvZ/OmpR TCS, known for its role in osmolarity/pH-dependent regulation of outer membrane porins across bacterial species, is also a central virulence regulator. However, the environmental cues that activate EnvZ/OmpR to trigger pathogenicity have remained unclear, limiting our understanding of host-pathogen interactions. Here, we demonstrate that in Vibrio parahaemolyticus, a major etiological agent of seafood-associated gastroenteritis, EnvZ functions as a direct ferric iron (Fe3+) sensor governing virulence programs. Fe3+-EnvZ interaction triggers kinase phosphorylation and activation, enabling transcriptional control of biofilm formation, swarming motility, and type 3/6 secretion systems. An iron-binding-deficient EnvZ mutant (EnvZQ103A) abrogated Fe3+ responsiveness and downstream signaling pathways. In an infant rabbit infection model, Fe3+ enhanced V. parahaemolyticus intestinal colonization and virulence through EnvZ/OmpR signaling. This study identifies Fe3+ as the physiological ligand activating the EnvZ/OmpR virulence regulon and provides insight into how enteric pathogens exploit host-derived iron cues to promote infection.
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