| RRC ID |
87940
|
| Author |
Shimada S, Aoki K, Shiozawa A, Nakai R, Fujiwara N, Komori K, Kudoh S, Imura S, Ishii Y, Tateda K.
|
| Title |
Characterization of Mycobacterium antarcticum sp. nov., a psychrotolerant member of the genus Mycobacterium isolated from Antarctic soil.
|
| Journal |
Sci Rep
|
| Abstract |
Most species of the genus Mycobacterium are widely distributed in soil and aquatic environments; however, there is limited understanding of their distribution and adaptation to permanently cold environments. Psychrophilic/psychrotolerant bacteria isolated from cold environments represent an important resource that can be used to understand these aspects. Here, we report representative psychrotolerant mycobacteria strains, designated TUM20985T, TUM20983, and TUM20984, from Antarctic soils. Phylogenetic analysis using the 16S rRNA gene and average nucleotide identity confirmed that these three strains belong to the same species, and are most closely related to Mycobacterium hodleri JCM 12141T. These strains are psychrotolerant and grow at 4-25 °C, but not above 30 °C. All three strains exhibited a higher proportion of unsaturated fatty acids compared with closely related species, with C18:1ω9c, C17:1ω8c, C17:0, and C16:0 identified as the major fatty acids. The predominant respiratory quinone was MK-9(H2). Comparative genomic analysis showed potential functional divergence in lipid metabolism genes, and revealed the presence of nitrate reduction and potential methane or ammonia oxidation genes that were absent in closely related species. Based on these findings, we propose that our strains represent a novel species, designated Mycobacterium antarcticum sp. nov., with TUM20985T (RIMD 4000203T = NCTC 15058T) as the type strain.
|
| Volume |
15(1)
|
| Pages |
34231
|
| Published |
2025-10-1
|
| DOI |
10.1038/s41598-025-16147-6
|
| PII |
10.1038/s41598-025-16147-6
|
| PMID |
41034288
|
| PMC |
PMC12489007
|
| MeSH |
Antarctic Regions
Cold Temperature
DNA, Bacterial / genetics
Fatty Acids / analysis
Genome, Bacterial
Mycobacterium* / classification
Mycobacterium* / genetics
Mycobacterium* / isolation & purification
Phylogeny
RNA, Ribosomal, 16S / genetics
Soil Microbiology*
|
| IF |
3.998
|
| Resource |
| Pathogenic bacteria |
|
| General Microbes |
JCM13574
JCM12141 |
