論文 - 詳細
| RRC ID | 87954 |
|---|---|
| 著者 | Lizama CO, Hawkins JS, Schmitt CE, Bos FL, Zape JP, Cautivo KM, Borges Pinto H, Rhyner AM, Yu H, Donohoe ME, Wythe JD, Zovein AC. |
| タイトル | Repression of arterial genes in hemogenic endothelium is sufficient for haematopoietic fate acquisition. |
| ジャーナル | Nat Commun |
| Abstract |
Changes in cell fate and identity are essential for endothelial-to-haematopoietic transition (EHT), an embryonic process that generates the first adult populations of haematopoietic stem cells (HSCs) from hemogenic endothelial cells. Dissecting EHT regulation is a critical step towards the production of in vitro derived HSCs. Yet, we do not know how distinct endothelial and haematopoietic fates are parsed during the transition. Here we show that genes required for arterial identity function later to repress haematopoietic fate. Tissue-specific, temporally controlled, genetic loss of arterial genes (Sox17 and Notch1) during EHT results in increased production of haematopoietic cells due to loss of Sox17-mediated repression of haematopoietic transcription factors (Runx1 and Gata2). However, the increase in EHT can be abrogated by increased Notch signalling. These findings demonstrate that the endothelial haematopoietic fate switch is actively repressed in a population of endothelial cells, and that derepression of these programs augments haematopoietic output. |
| 巻・号 | 6 |
| ページ | 7739 |
| 公開日 | 2015-7-23 |
| DOI | 10.1038/ncomms8739 |
| PII | ncomms8739 |
| PMID | 26204127 |
| PMC | PMC4519987 |
| MeSH | Animals Blood Vessels / embryology* Core Binding Factor Alpha 2 Subunit / metabolism* Female GATA2 Transcription Factor / metabolism* Genes, Reporter HMGB Proteins / physiology* Hemangioblasts / physiology* Hematopoiesis Mice Pregnancy Receptor, Notch1 / metabolism SOXF Transcription Factors / physiology* |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC06137 |