| RRC ID |
88013
|
| 著者 |
Jakovljević S, Radojević D, Bajić SS, Brdarić E, Mitrović H, Đokić J, Baumann L, Trifunovic A, Golić N, Dinić M.
|
| タイトル |
Bacterial exopolysaccharides mediate activation of flavin-containing monooxygenase 2 to extend lifespan in Caenorhabditis elegans.
|
| ジャーナル |
Cell Commun Signal
|
| Abstract |
The gut microbiota plays a pivotal role in modulating host physiology and longevity through the production of microbial-derived molecules. Among these, bacterial exopolysaccharides (EPS) represent a structurally diverse group of surface polysaccharides with emerging roles in regulating host well-being. Here, we investigated the role of Lactobacillus strains with the capability to produce EPS, using Caenorhabditis elegans as a model organism. Results revealed significant lifespan extension in worms fed with EPS-producing bacteria, accompanied by improved health-span markers such as enhanced pharyngeal pumping and reduced lipofuscin accumulation. Transcriptomic profiling identified robust upregulation of the host detoxification and immune defense pathways, highlighting the flavin-containing monooxygenase gene fmo-2, as one of the major mediators of longevity and stress resistance triggered by EPS-producing lactobacilli. The effect was confirmed using fmo-2p::GFP reporter animals and was abrogated in fmo-2, hlh-30, and nhr-49 mutant backgrounds. Mechanistically, we demonstrated that EPS acts through a conserved transcriptional network that primarily relies on the activation of nhr-49/PPAR-α, with purified EPS being sufficient to activate fmo-2 expression. Our findings reveal that bacterial EPS activates host xenobiotic pathways to modulate aging, positioning it as a potential tool for microbiota-based longevity interventions. These insights show how microbial products can modulate fundamental biological processes across species, opening new strategies for age-related health interventions.
|
| 巻・号 |
24(1)
|
| ページ |
9
|
| 公開日 |
2025-11-29
|
| DOI |
10.1186/s12964-025-02567-4
|
| PII |
10.1186/s12964-025-02567-4
|
| PMID |
41318463
|
| PMC |
PMC12781332
|
| MeSH |
Animals
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / microbiology
Caenorhabditis elegans* / physiology
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans Proteins* / metabolism
Longevity* / drug effects
Oxygenases* / genetics
Oxygenases* / metabolism
Polysaccharides, Bacterial* / metabolism
Polysaccharides, Bacterial* / pharmacology
|
| リソース情報 |
| 線虫 |
tm1978 |
