Reference - Detail
| RRC ID | 88035 |
|---|---|
| Author | Kalugotla G, Marmerstein V, Schriefer LA, Wang L, Morrison SA, Perez LC, Silverman GA, Schedl T, Pak SC, Baldridge MT. |
| Title | ATG-3 limits Orsay virus infection in C. elegans and regulates collagen pathways. |
| Journal | PLoS Pathog |
| Abstract |
Autophagy is an essential cellular process which functions to maintain homeostasis in response to stressors such as starvation or infection. Here, we report that a subset of autophagy factors including ATG-3 play an antiviral role in Orsay virus infection of Caenorhabditis elegans. Orsay virus infection does not modulate autophagic flux, and re-feeding after starvation limits Orsay virus infection and blocks autophagic flux, suggesting that the role of ATG-3 in Orsay virus susceptibility is independent of its role in maintaining autophagic flux. atg-3 mutants phenocopy rde-1 mutants, which have a defect in RNA interference (RNAi), in susceptibility to Orsay virus infection and transcriptional response to infection. However, atg-3 mutants do not exhibit defects in RNAi. Additionally, ATG-3 limits viral infection at a post-entry step, similar to RDE-1. Differential expression analysis using RNA sequencing revealed that antiviral sqt-2, which encodes a collagen trimer protein, is depleted in mock-infected and infected atg-3 mutants, as well as in infected WT animals, as are numerous other collagen genes. These data suggest that ATG-3 may have a role in collagen organization pathways that function in antiviral defense in C. elegans. |
| Volume | 21(11) |
| Pages | e1012900 |
| Published | 2025-11-1 |
| DOI | 10.1371/journal.ppat.1012900 |
| PII | PPATHOGENS-D-25-00097 |
| PMID | 41252446 |
| PMC | PMC12646485 |
| MeSH | Animals Autophagy Autophagy-Related Proteins* / genetics Autophagy-Related Proteins* / metabolism Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans* / virology Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Collagen* / genetics Collagen* / metabolism Nodaviridae* RNA Virus Infections* / immunology RNA Virus Infections* / metabolism |
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| The most frequently cited source | Blogs |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm3244 |