| RRC ID |
88053
|
| 著者 |
Wang R, Tian Y, Liu L, Wang JH, Zhao L, Liu Y, Qi F, Zhang L, Zhou J, Dong MQ, Gao J.
|
| タイトル |
A DNA-containing phase-separated compartment links the nuclear envelope to chromosome axes to promote homolog pairing in C. elegans.
|
| ジャーナル |
Sci Adv
|
| Abstract |
Homologous chromosome pairing is a critical prerequisite for successful meiosis, yet the molecular mechanisms underlying this process remain elusive. In Caenorhabditis elegans, chromosome pairing centers (PCs) and PC-recruited proteins mediate homolog recognition and pairing. Here, we further dissect the molecular characteristics of this system and reveal the involvement of a phase-separated compartment in pairing. Focusing on the PC protein HIM-8, we explored its functional domains in vivo and its self-multimerizing properties in vitro. We find that the amino-terminal domain facilitates PC chromatin binding of the carboxyl-terminal domain, whereas the middle unstructured region is essential for PC association with the nuclear envelop (NE). Integrated analyses suggest that PC-NE association is mediated by a DNA-containing phase-separated compartment that is spatially distinct from, but connected to, the chromosome axis-likely through flexible linkages. We propose that homology evaluation occurs within this compartment, where direct DNA-DNA interactions could play a role.
|
| 巻・号 |
11(41)
|
| ページ |
eadw5764
|
| 公開日 |
2025-10-10
|
| DOI |
10.1126/sciadv.adw5764
|
| PMID |
41071891
|
| PMC |
PMC12513456
|
| MeSH |
Animals
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / metabolism
Caenorhabditis elegans Proteins* / chemistry
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans Proteins* / metabolism
Cell Cycle Proteins
Chromatin / metabolism
Chromosome Pairing*
Chromosomes*
DNA* / metabolism
Meiosis / genetics
Nuclear Envelope* / genetics
Nuclear Envelope* / metabolism
Protein Binding
|
| リソース情報 |
| 線虫 |
tm3655 |
