論文 - 詳細
| RRC ID | 88053 |
|---|---|
| 著者 | Wang R, Tian Y, Liu L, Wang JH, Zhao L, Liu Y, Qi F, Zhang L, Zhou J, Dong MQ, Gao J. |
| タイトル | A DNA-containing phase-separated compartment links the nuclear envelope to chromosome axes to promote homolog pairing in C. elegans. |
| ジャーナル | Sci Adv |
| Abstract |
Homologous chromosome pairing is a critical prerequisite for successful meiosis, yet the molecular mechanisms underlying this process remain elusive. In Caenorhabditis elegans, chromosome pairing centers (PCs) and PC-recruited proteins mediate homolog recognition and pairing. Here, we further dissect the molecular characteristics of this system and reveal the involvement of a phase-separated compartment in pairing. Focusing on the PC protein HIM-8, we explored its functional domains in vivo and its self-multimerizing properties in vitro. We find that the amino-terminal domain facilitates PC chromatin binding of the carboxyl-terminal domain, whereas the middle unstructured region is essential for PC association with the nuclear envelop (NE). Integrated analyses suggest that PC-NE association is mediated by a DNA-containing phase-separated compartment that is spatially distinct from, but connected to, the chromosome axis-likely through flexible linkages. We propose that homology evaluation occurs within this compartment, where direct DNA-DNA interactions could play a role. |
| 巻・号 | 11(41) |
| ページ | eadw5764 |
| 公開日 | 2025-10-10 |
| DOI | 10.1126/sciadv.adw5764 |
| PMID | 41071891 |
| PMC | PMC12513456 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / chemistry Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cell Cycle Proteins Chromatin / metabolism Chromosome Pairing* Chromosomes* DNA* / metabolism Meiosis / genetics Nuclear Envelope* / genetics Nuclear Envelope* / metabolism Protein Binding |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
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| 線虫 | tm3655 |