| RRC ID |
88056
|
| Author |
Serot C, Scarcelli V, Pouget A, Largeau C, Sagot A, El-Hachami K, Dupuy D, Culetto E, Lefebvre C, Legouis R.
|
| Title |
Reticulon-dependent ER-phagy mediates adaptation to heat stress in C. elegans.
|
| Journal |
Curr Biol
|
| Abstract |
The selective degradation of endoplasmic reticulum (ER) by autophagy, named ER-phagy, promotes the recovery of ER homeostasis after stress. Depending on the ER stress, different types of ER-phagy involve various selective autophagy receptors. In this study, we report a macroER-phagy induced by the fragmentation of tubular ER in response to acute heat stress. We identified a novel ER-phagy receptor encoded by the reticulon long isoform RET-1d. RET-1d is mainly expressed in the nervous system and the epidermis and colocalizes with the ubiquitin-like autophagy protein LGG-1/GABARAP during heat-stress-induced autophagy. Two LC3-interacting region (LIR) motifs in the long intrinsically disordered region of RET-1d mediate its interaction with the LGG-1 protein. The specific depletion of the RET-1d isoform or the mutations of the LIRs resulted in a defective ER-phagy and a decrease in the capacity of animals to adapt to heat stress. Our data revealed a RET-1d- and LGG-1-dependent ER-phagy mechanism that takes place in neurons and epidermis and participates in the adaptation of C. elegans to heat stress.
|
| Volume |
35(10)
|
| Pages |
2365-2378.e7
|
| Published |
2025-5-19
|
| DOI |
10.1016/j.cub.2025.04.028
|
| PII |
S0960-9822(25)00457-9
|
| PMID |
40328253
|
| MeSH |
Adaptation, Physiological
Animals
Autophagy* / physiology
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / physiology
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans Proteins* / metabolism
Endoplasmic Reticulum* / metabolism
Endoplasmic Reticulum Stress*
Heat-Shock Response* / physiology
|
| Resource |
| C.elegans |
tm390 |
