論文 - 詳細
| RRC ID | 88076 |
|---|---|
| 著者 | Pastore B, Hertz HL, Tang W. |
| タイトル | The ribonuclease DISL-2 mediates piRNA degradation in C. elegans. |
| ジャーナル | Cell Rep |
| Abstract |
The PIWI/piRNA pathway acts as a conserved RNA-directed immune mechanism that suppresses transposons and promotes fertility. The biogenesis of piRNAs has been extensively investigated, yet the mechanisms governing piRNA degradation remain poorly understood. Here, we systematically measure the half-lives of thousands of Caenorhabditis elegans piRNAs, revealing a broad range from less than 1 h to over 20 h. Notably, piRNAs with 3' terminal uridines (U) degrade faster than those ending in 3' guanines or cytosines, suggesting that terminal nucleotides modulate piRNA stability. We identify DISL-2, a 3' to 5' exoribonuclease as a key enzyme for degrading piRNAs with templated 3' uridines and non-templated 3' uridines, the latter catalyzed by poly(U) polymerase PUP-1. Furthermore, DISL-2 acts as a quality control factor to target piRNAs that are improperly processed. Together, these findings advance our understanding of piRNA homeostasis by determining piRNA half-lives and identifying factors responsible for piRNA decay. |
| 巻・号 | 44(10) |
| ページ | 116430 |
| 公開日 | 2025-10-28 |
| DOI | 10.1016/j.celrep.2025.116430 |
| PII | S2211-1247(25)01201-X |
| PMID | 41075248 |
| PMC | PMC12588376 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Exoribonucleases* / genetics Exoribonucleases* / metabolism Piwi-Interacting RNA RNA Stability RNA, Small Interfering* / genetics RNA, Small Interfering* / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm5337 tm1021 tm872 tm4477 tm4344 tm869 tm5089 |