Reference - Detail
| RRC ID | 88095 |
|---|---|
| Author | Agostini L, Pfister JA, Basnet N, Ding J, Zhang R, Biertümpfel C, O'Connell KF, Mizuno N. |
| Title | Structural insights into SSNA1 self-assembly and its microtubule binding for centriole maintenance. |
| Journal | Nat Commun |
| Abstract |
SSNA1 is a fibrillar protein involved in dynamic microtubule remodeling, including nucleation, co-polymerization, and microtubule branching. The underlying molecular mechanism has remained unclear due to a lack of structural information. Here, we determine the cryo-EM structure of C.elegans SSNA-1 at 4.55-Å resolution and evaluate its role in embryonic development. We find that SSNA-1 forms an anti-parallel coiled-coil, with self-assembly facilitated by an overhang of 16 C-terminal residues that form a triple-stranded helical junction. The microtubule-binding region is within the triple-stranded junction, suggesting that self-assembly of SSNA-1 creates hubs for effective microtubule interaction. Genetical analysis elucidates that SSNA-1 deletion significantly reduces embryonic viability, and causes multipolar spindles during cell division. Interestingly, impairing SSNA-1 self-assembly has a comparable effect on embryonic viability as the knockout strain. Our study provides molecular insights into SSNA-1's self-assembly and its role in microtubule binding and cell division regulation through centriole stability. |
| Volume | 16(1) |
| Pages | 7512 |
| Published | 2025-8-13 |
| DOI | 10.1038/s41467-025-62696-9 |
| PII | 10.1038/s41467-025-62696-9 |
| PMID | 40804232 |
| PMC | PMC12350680 |
| MeSH | Animals Caenorhabditis elegans* / embryology Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Cell Division Centrioles* / metabolism Cryoelectron Microscopy Embryonic Development Microtubules* / metabolism Microtubules* / ultrastructure Protein Binding |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Bluesky |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm9713 tmIs1245 |