論文 - 詳細
| RRC ID | 88110 |
|---|---|
| 著者 | Nikonorova IA, desRanleau E, Jacobs KC, Saul J, Walsh JD, Wang J, Barr MM. |
| タイトル | Polycystins recruit cargo to distinct ciliary extracellular vesicle subtypes in C. elegans. |
| ジャーナル | Nat Commun |
| Abstract |
Therapeutic use of tiny extracellular vesicles (EVs) requires understanding cargo loading mechanisms. Here, we use a modular proximity labeling approach to identify the cargo of ciliary EVs associated with the transient receptor potential channel polycystin-2 PKD-2 of C. elegans. Polycystins are conserved ciliary proteins and cargo of EVs; dysfunction causes polycystic kidney disease in humans and mating deficits in C. elegans. We discover that polycystins localize with specific cargo on ciliary EVs: polycystin-associated channel-like protein PACL-1, dorsal and ventral polycystin-associated membrane C-type lectins PAMLs, and conserved tumor necrosis factor receptor-associated factor (TRAF) TRF-1 and TRF-2. Loading of these components to EVs relies on polycystin-1 LOV-1. Our modular EV-TurboID approach can be applied in both cell- and tissue-specific manners to define the composition of distinct EV subtypes, addressing a major challenge of the EV field. |
| 巻・号 | 16(1) |
| ページ | 2899 |
| 公開日 | 2025-4-3 |
| DOI | 10.1038/s41467-025-57512-3 |
| PII | 10.1038/s41467-025-57512-3 |
| PMID | 40180912 |
| PMC | PMC11968823 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cilia* / metabolism Extracellular Vesicles* / metabolism Humans Lectins, C-Type / metabolism TRPP Cation Channels* / genetics TRPP Cation Channels* / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 37 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm12527 tm12580 tm5167 |