Reference - Detail
| RRC ID | 88118 |
|---|---|
| Author | Mahapatra A, Mohankumar M, Hundley HA. |
| Title | The kinase GSK-3 alters the RNA-binding protein landscape of lipid metabolism transcripts leading to altered expression in the C. elegans nervous system. |
| Journal | Nucleic Acids Res |
| Abstract |
Tissue-specific regulation of gene expression is essential for multicellular organisms, and RNA-binding proteins play central roles in these molecular processes. To determine how the Caenorhabditis elegans RNA-binding protein, ADR-1, regulates tissue-specific gene expression, we profiled the RNA-binding targets of ADR-1 in neural cells and assessed the effects of ADR-1 binding on neural gene expression. We identified a cohort of neural transcripts that function in lipid metabolism and are directly regulated by ADR-1 binding. To identify cellular factors that influence ADR-1 binding, a forward genetic screen was performed, revealing that the serine/threonine protein kinase, glycogen synthase kinase-3 (GSK-3), inhibits ADR-1 binding to the cohort. Further investigation revealed that the RNA-binding protein VIG-1 physically interacts with ADR-1, and the two proteins coordinately bind the neural lipid metabolism transcripts. Additional experiments revealed that VIG-1 is phosphorylated in a GSK-3-dependent manner, which inhibits the VIG-1-ADR-1 complex from binding the regulon in wild-type animals. Importantly, inhibition of GSK-3 kinase activity in wild-type animals also resulted in decreased neural expression of lipid metabolism genes. Together, we reveal that the interplay between a kinase and RNA-binding proteins regulates the expression of lipid metabolism genes within neural cells, potentially impacting stress resistance and longevity. |
| Volume | 53(15) |
| Published | 2025-8-11 |
| DOI | 10.1093/nar/gkaf785 |
| PII | 8237890 |
| PMID | 40829802 |
| PMC | PMC12362252 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Gene Expression Regulation Glycogen Synthase Kinase 3* / genetics Glycogen Synthase Kinase 3* / metabolism Lipid Metabolism* / genetics Nervous System* / metabolism Neurons / metabolism Phosphorylation Protein Binding RNA-Binding Proteins* / genetics RNA-Binding Proteins* / metabolism |
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オルトメトリクス指標項目
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| The most frequently cited source | Bluesky |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm668 |