RRC ID 88168
Author Balachandar Thendral S, Roos N, Vertiz J, S Wokasch A, Maier P, R Sherwood D.
Title Time-lapse imaging of C. elegans egg-laying with high spatiotemporal resolution.
Journal MicroPubl Biol
Abstract Egg-laying behavior in C. elegans is driven by a simple neural circuit comprising motor neurons, vulva muscles, and neuroendocrine cells. Most protocols designed to capture this behavior rely on imaging freely moving animals at low magnifications, thus limiting subcellular-level understanding of the underlying neuromuscular circuit. Here, we present a protocol to immobilize C. elegans while maintaining egg-laying activity, enabling high spatiotemporal-resolution imaging of this behavior.
Volume 2025
Published 2025-1-1
DOI 10.17912/micropub.biology.001914
PMID 41426963
PMC PMC12714348
Resource
C.elegans tm927