| RRC ID |
88179
|
| Author |
Rei Yagasaki, Ryo Nakamura, Yuuki Shikaya, Ryosuke Tadokoro, Ruolin Hao, Zhe Wang, Mototsugu Eiraku, Masafumi Inaba, Yoshiko Takahashi
|
| Title |
The gut contractile organoid for studying the gut motility regulated by coordinating signals between interstitial cells of Cajal and smooth muscles
|
| Journal |
eLife
|
| Abstract |
The gut undergoes peristaltic movements regulated by intricate cellular interactions. How these interactions emerge in the developing gut remains poorly explored due to a lack of model system. We here developed a novel contractile organoid that is derived from the muscle layer of chicken embryonic hindgut. The organoid contained smooth muscle cells (SMCs) and interstitial cells of Cajal (ICCs; pacemaker) with few enteric neurons and underwent periodic contractions. The organoid was formed by self-organization with morphological arrangements of ICCs (internal) and SMCs (peripheral), allowing identification of these cells in live. GCaMP-Ca
2+
imaging analyses revealed that Ca
2+
transients between ICC-ICC, SMC-SMC, or SMC-ICC were markedly coordinated. Pharmacological studies further suggested a role of gap junctions in ICC-to-SMC signaling, and also possible mechanical feedback from SMC’s contraction to ICC’s pace-making activities. In addition, two organoids with different rhythms became synchronized when mediated by SMCs, unveiling a novel contribution of SMCs to ICC’s pace-making. The gut contractile organoid developed in this study offers a useful model to understand how the rhythm coordination between/among ICCs and SMCs is regulated and maintained during gut development.
|
| Volume |
13
|
| Published |
2025-8-22
|
| DOI |
10.7554/elife.97860.3
|
| PMID |
40843588
|
| PMC |
PMC12373395
|
| MeSH |
Animals
Calcium / metabolism
Cell Communication
Chick Embryo
Chickens
Gastrointestinal Motility* / physiology
Interstitial Cells of Cajal* / physiology
Muscle Contraction*
Muscle, Smooth* / physiology
Myocytes, Smooth Muscle* / physiology
Organoids* / physiology
Signal Transduction
|
| Resource |
| Chicken / Quail |
|
