論文 - 詳細
| RRC ID | 88211 |
|---|---|
| 著者 | Tohmi M, Namba H, Kawamura M, Hishida R, Iguchi S, Kobayashi K, Matsushita N, Mashimo T, Natsume R, Sakimura K, Nawa H. |
| タイトル | Transcranial Calcium Macro-Imaging From the Auditory Cortex of Thy1-Cre-Driven GCaMP8 Transgenic Rats. |
| ジャーナル | Neuropsychopharmacol Rep |
| Abstract |
BACKGROUND:Genetically encoded calcium indicators such as GCaMP enable us to monitor neuronal activities from living animals at synaptic levels as well as at whole-brain levels. However, the genetic variation of GCaMP transgenic rats is limited compared with the various demands for neuroscience studies on rats. METHOD:We established two transgenic rat lines: one expressing Cre recombinase under the control of the Thy1 promoter and another knock-in line carrying a floxed STOP cassette followed by GCaMP8 inserted into the β-actin locus. We mated these two transgenic rats and produced the Thy1-Cre-driven GCaMP8-expressing rats. RESULTS:Approximately 80% of cortical neurons were positive for the GCaMP immunoreactivity. The GCaMP8-expressing rats were subjected to transcranial calcium imaging from the auditory cortex under anesthesia. With this simple procedure, we were able to detect the maximum responses to 68 dB click sounds;ΔF/F0 = 1.2%. The rise time reaching the peak response was approximately 150 ms, and the half decay time was about 150 ms. CONCLUSIONS:These results suggest that the present GCaMP8-expressing rats as well as their parental transgenic rat strains offer a new tool for cortical macro-imaging and functional mapping with the transcranial approach. |
| 巻・号 | 46(2) |
| ページ | e70113 |
| 公開日 | 2026-6-1 |
| DOI | 10.1002/npr2.70113 |
| PMID | 42050644 |
| PMC | PMC13124658 |
| MeSH | Acoustic Stimulation Animals Auditory Cortex* / metabolism Auditory Cortex* / physiology Calcium* / metabolism Female Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Integrases / genetics Integrases / metabolism Male Neurons / metabolism Rats Rats, Transgenic Thy-1 Antigens* / genetics Thy-1 Antigens* / metabolism |
| リソース情報 | |
| ラット | SD.LIS-Actbtm1(CAG-GCaMP8)Ksak (StrainID=1372) SD-Tg(Thy1-cre)18Larc |