論文 - 詳細
| RRC ID | 88217 |
|---|---|
| 著者 | Esquinas-Ariza RM, Villar I, Minguillón S, Zamarreño Á, Pérez-Rontomé C, Reeder BJ, Sandal N, Yan D, García-Mina JM, Duanmu D, Martínez-Júlvez M, Becana M. |
| タイトル | Structural and functional comparison of hemoglobin Glb2-1 of Lotus japonicus with Glb1-1 and leghemoglobins. |
| ジャーナル | J Exp Bot |
| Abstract |
The legume Lotus japonicus expresses nine hemoglobins, including leghemoglobins (Lbs), class 1 phytoglobin (Glb1-1), and an unusual phytoglobin (Glb2-1). Quantitative PCR, proteomics, and plant mutant analyses indicated that Glb2-1 is mainly present in nodules without replacing Lb function, but is also in roots and photosynthetic tissues. Comparison of hormonal profiles of the knock-out mutants glb1-1, glb2-1, and glb1-1/2-1 showed that Glb1-1 and Glb2-1 have distinct functions. The increase of salicylic acid in the leaves of glb1-1 revealed a role of Glb1-1 in the defense response, which was corroborated by accumulation of pipecolic acid, a metabolite involved in plant immunity. In contrast, the decrease of bioactive jasmonoyl-isoleucine in glb2-1 was consistent with a role of Glb2-1 in the plant's reproductive stage. The mutants also showed changes in cytokinins, gibberellins, and polyamines, but without clear distinctive patterns. The crystal structure of Glb2-1 was determined to 1.6 Å resolution and compared with those of soybean Lba and Arabidopsis Glb1. In combination with mutant versions of Glb2-1, residues Tyr31, His64, and Cys65 were identified as critical for O2-binding stability. Spectral changes in heme coordination when Tyr31 is substituted for Phe highlights the importance of the residue at the B10 position for Lb and Glb function. |
| 巻・号 | 77(2) |
| ページ | 511-527 |
| 公開日 | 2026-1-12 |
| DOI | 10.1093/jxb/eraf434 |
| PII | 8268508 |
| PMID | 41025407 |
| PMC | PMC12794238 |
| MeSH | Hemoglobins* / chemistry Hemoglobins* / genetics Hemoglobins* / metabolism Leghemoglobin* / chemistry Leghemoglobin* / genetics Leghemoglobin* / metabolism Lotus* / chemistry Lotus* / genetics Lotus* / metabolism Plant Proteins* / chemistry Plant Proteins* / genetics Plant Proteins* / metabolism |
| IF | 5.908 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ミヤコグサ・ダイズ | Gifu B-129 MG-20 |