| RRC ID |
88252
|
| Author |
Kuwabara K, Gaetan VBA, Nakajima R, Ezura K, Toriyama K, Shirasawa K, Ariizumi T.
|
| Title |
Fertility restoration in cytoplasmic male sterile tomato via knockout of either DNA polymerase I or Topoisomerase I, two nuclear-encoded organellar DNA replication genes.
|
| Journal |
Plant Physiol Biochem
|
| Abstract |
Cytoplasmic male sterility (CMS) and restorer-of-fertility (Rf) genes are widely used for F1 hybrid breeding. To date, most identified Rf genes encode pentatricopeptide repeat (PPR) proteins, whereas several Rf genes encode other proteins. Identification of Rf genes is essential for promoting F1 hybrid breeding; however, exploring Rf genes that encode proteins other than PPR is challenging because information on such genes remains limited. In this study, we focused on the nuclear genes involved in mitochondrial DNA replication to identify novel tomato Rf genes. Twenty-six tomato genes homologous to 19 Arabidopsis mitochondrial DNA replication genes were screened for mutations using whole-genome sequencing data from fertility-restored tomato suppressor mutants, and several candidate mutations for fertility restoration were identified. CRISPR/Cas9-mediated gene knockout experiments revealed that the loss of function of SlPolI, which encodes an organellar DNA polymerase, and SlTopI, which encodes an organellar topoisomerase I, restored pollen fertility in tomato CMS lines. This study provides new insights into the molecular basis of fertility restoration and opens up opportunities for the efficient discovery of Rf genes to accelerate F1 hybrid breeding in tomatoes and other crops.
|
| Volume |
230
|
| Pages |
110970
|
| Published |
2026-1-1
|
| DOI |
10.1016/j.plaphy.2025.110970
|
| PII |
S0981-9428(25)01498-6
|
| PMID |
41421144
|
| MeSH |
Cell Nucleus / genetics
Cell Nucleus / metabolism
DNA Replication* / genetics
DNA Topoisomerases, Type I* / genetics
DNA Topoisomerases, Type I* / metabolism
DNA-Directed DNA Polymerase* / genetics
DNA-Directed DNA Polymerase* / metabolism
Fertility / genetics
Gene Knockout Techniques
Plant Infertility* / genetics
Plant Proteins* / genetics
Plant Proteins* / metabolism
Pollen / genetics
Solanum lycopersicum* / genetics
Solanum lycopersicum* / physiology
|
| Resource |
| Tomato |
|
