Reference - Detail
| RRC ID | 88256 |
|---|---|
| Author | Mountourakis F, Fragkostefanakis S, Moschou PN. |
| Title | Protocol for generating and selecting transgenic tomato lines through Agrobacterium tumefaciens-mediated transformation. |
| Journal | STAR Protoc |
| Abstract |
Here, we present a protocol for generating and selecting stable transgenic tomato lines through Agrobacterium tumefaciens-mediated transformation of cotyledon and hypocotyl explants. We describe steps for sterilizing and planting tomato seeds, cotyledon and hypocotyl excision and preculture, Agrobacterium tumefaciens preparation and co-cultivation with explants, and recovering explants and bacterial overgrowth restriction. We then detail procedures for selecting green calli; regenerating explant excision and growth in non-selective media; plant hardening, potting, and growing; and T-DNA insertion confirmation by PCR. For complete details on the use and execution of this protocol, please refer to Mountourakis et al.1,2,3. |
| Volume | 7(1) |
| Pages | 104322 |
| Published | 2026-3-20 |
| DOI | 10.1016/j.xpro.2025.104322 |
| PII | S2666-1667(25)00728-2 |
| PMID | 41520340 |
| PMC | PMC12819032 |
| MeSH | Agrobacterium tumefaciens* / genetics Cotyledon / genetics Hypocotyl / genetics Plants, Genetically Modified* / genetics Plants, Genetically Modified* / growth & development Solanum lycopersicum* / genetics Solanum lycopersicum* / growth & development Transformation, Genetic* |
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Tomato | TOMJPF00001 |