| RRC ID |
88301
|
| Author |
Willetts L, Felix LC, Jacobsen EA, Puttagunta L, Condjella RM, Zellner KR, Ochkur SI, Kim JD, Luo H, Lee NA, Lee JJ, Moqbel R, Lacy P.
|
| Title |
Vesicle-associated membrane protein 7-mediated eosinophil degranulation promotes allergic airway inflammation in mice.
|
| Journal |
Commun Biol
|
| Abstract |
Eosinophil degranulation is a determining factor in allergy-mediated airway pathology. Receptor-mediated degranulation in eosinophils requires vesicle-associated membrane protein 7 (VAMP-7), a principal component of the SNARE fusion machinery. The specific contribution of eosinophil degranulation to allergen-induced airway responses remains poorly understood. We generated mice with VAMP-7 gene deficiency exclusively in eosinophils (eoCRE/V7) from a cross using eosinophil-specific Cre recombinase-expressing mice crossed with VAMP-7f/f mice. Eosinophils from eoCRE/V7 mice showed deficient degranulation responses in vitro, and responses continued to be decreased following ex vivo intratracheal adoptive transfer of eoCRE/V7 eosinophils into IL-5/hE2/EPX-/- mice. Consistent with diminished degranulation responses, reduced airway hyperresponsiveness was observed in ovalbumin-sensitized and challenged eoCRE/V7 mice following methacholine inhalation. Therefore, VAMP-7 mediates eosinophil degranulation both in vitro and ex vivo, and this event augments airway hyperresponsiveness.
|
| Volume |
1
|
| Pages |
83
|
| Published |
2018-1-1
|
| DOI |
10.1038/s42003-018-0081-z
|
| PII |
10.1038/s42003-018-0081-z
|
| PMID |
30271964
|
| PMC |
PMC6123774
|
| Resource |
| Mice |
RBRC01926 |
