| RRC ID |
88367
|
| 著者 |
Kohji Ishihara, Noriyoshi Masuoka, Hiroki Hamada, Kei Shimoda
|
| タイトル |
Biocatalytic Ability of the Hairy Bracket Mushroom (Trametes hirsuta): Bio-reduction of Carbonyl Compounds
|
| ジャーナル |
International Journal of Current Microbiology and Applied Sciences
|
| Abstract |
This study screened the potential biocatalytic abilities of four hairy bracket mushroom (Trametes strains) strains. Commercially available potato dextrose broth (PDB) and a medium recommended by the Fungus/Mushroom Resource and Research Center (MA medium) were used for culturing these mushrooms. Good growth was observed in two strains –TUFC13731 and 31398– cultured using the PDB. The stereoselective reduction of α- and β-keto esters by these strains was investigated, and they were found to reduce various α-keto esters. Specifically, the reduction of α-keto esters by the PDB-cultivated T. hirsuta TUFC13731 strain in the presence of L-glutamate as an additive yielded the corresponding α-hydroxy esters with a high conversion ratio and excellent enantioselectivity. Furthermore, this strain stereospecifically reduced ethyl 2-methyl-3-oxobutanoate to (2S, 3S)-2-methyl-3-hydroxybutanoate, i.e., only one of the four theoretically possible isomers. Overall, hairy bracket mushrooms demonstrated great potential for application as biocatalysts for the stereoselective reduction of carbonyl compounds. The present study results also suggest that cryopreserved T. hirsuta cells can be used as biocatalysts for the asymmetric reduction of carbonyl compounds.
|
| 巻・号 |
15
|
| ページ |
40-47
|
| 公開日 |
2026-1-20
|
| DOI |
10.20546/ijcmas.2026.1501.004
|
| リソース情報 |
| きのこ |
TUFC 13731
TUFC 31398
TUFC 31400
TUFC 31401 |
