Reference - Detail
| RRC ID | 88384 |
|---|---|
| Author | Chen Y, Hata T, Rehman F, Kang L, Yang L, Kim BYS, Nguyen JH. |
| Title | Visualization of Hepatocellular Regeneration in Mice After Partial Hepatectomy. |
| Journal | J Surg Res |
| Abstract |
BACKGROUND:Although hepatocellular regeneration is the cornerstone of liver homeostasis, current techniques for assessing such regeneration are limited. A method for visualizing the regeneration process would provide a means for advanced studies. Therefore, we examined the possibility of using fluorescence ubiquination-based cell cycle indicator (Fucci) mice for direct visualization of hepatocellular regeneration. MATERIALS AND METHODS:We performed a two-thirds partial hepatectomy in conventional and Fucci mice. Fucci animals have orange Cdt1 expressed in the G1 phase and green Geminin expressed in S/G2/M phases. Regenerating livers were procured daily for 7 d. Immunohistochemical staining was performed for proliferative Ki67 and mitotic pHH3 serine 10 (pHH3) markers on formalin-fixed, paraffin-embedded tissue sections from conventional mice. The orange Cdt1 and green Geminin fluorescence indicative of the G1 and S/G2/M phases, respectively, were assessed in liver tissues, in vivo and ex vivo, with two-photon laser scanning microscopy. RESULTS:Immunostaining with Ki67 and pHH3 revealed a typical profile of hepatocellular regeneration after hepatectomy in conventional mice, although immunostaining required more than a week to process. In contrast, hepatocellular regeneration could be visualized with two-photon microscopy within a few hours in regenerating livers of the Fucci mice. Only orange G1 hepatocytes were seen in the baseline liver specimens; however, multiple bright green and yellow hepatocytes were seen 48 h after hepatectomy, indicating active hepatocytes in the S/G2/M phases of the cell cycle. CONCLUSIONS:Hepatocellular regeneration is readily visualized in regenerating livers of Fucci mice. The Fucci model is an exciting tool for advanced studies of hepatocellular and liver regeneration. |
| Volume | 235 |
| Pages | 494-500 |
| Published | 2019-3-1 |
| DOI | 10.1016/j.jss.2018.10.022 |
| PII | S0022-4804(18)30746-7 |
| PMID | 30691834 |
| PMC | PMC6355148 |
| MeSH | Animals Fluorescent Dyes* Hepatectomy Liver Regeneration* Male Mice, Inbred C57BL |
| Resource | |
| Mice | RBRC09613 |