論文 - 詳細
| RRC ID | 88451 |
|---|---|
| 著者 | Vujić N, Bradić I, Goeritzer M, Kuentzel KB, Rainer S, Kratky D, Radović B. |
| タイトル | ATG7 is dispensable for LC3-PE conjugation in thioglycolate-elicited mouse peritoneal macrophages. |
| ジャーナル | Autophagy |
| Abstract |
Thioglycolate-elicited macrophages exhibit abundant conjugation of LC3 with PE (LC3-II). Among other autophagy-related (ATG) proteins, it is proposed that, like in yeast, both ATG5 and ATG7 are essential for LC3 conjugation. Using atg5-deficient (-/-) and atg7-/-macrophages, we provide evidence that loss of ATG5 but not of ATG7 resulted in LC3-II depletion. Accumulation of LC3-II in elicited atg7-/- macrophages in response to bafilomycin A1 validated these data. Furthermore, complete loss of ATG3 in atg7-/- macrophages demonstrated that ATG7 and ATG3 are dispensable for LC3-PE conjugation. In contrast to thioglycolate-elicited macrophages, naïve peritoneal and bone marrow-derived atg7-/- macrophages exhibited no LC3-II, even under inflammatory stimuli in vitro. Hence, the macrophage metabolic status dictates the level of LC3-PE conjugation with a supportive but nonessential role of ATG7, disclosing the eukaryotic exception from the LC3 lipidation model based on yeast data. Abbreviations: ATG: autophagy-related; BM: bone marrow; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; PE: phosphatidylethanolamine. |
| 巻・号 | 17(11) |
| ページ | 3402-3407 |
| 公開日 | 2021-11-1 |
| DOI | 10.1080/15548627.2021.1874132 |
| PMID | 33459130 |
| PMC | PMC8632316 |
| MeSH | Animals Autophagy / physiology Autophagy-Related Protein 5 / deficiency Autophagy-Related Protein 5 / genetics Autophagy-Related Protein 5 / metabolism Autophagy-Related Protein 7 / deficiency Autophagy-Related Protein 7 / genetics Autophagy-Related Protein 7 / metabolism* Lipid Metabolism Macrolides / pharmacology Macrophages / drug effects Macrophages / metabolism Macrophages, Peritoneal / drug effects Macrophages, Peritoneal / metabolism* Mice Mice, Knockout Microtubule-Associated Proteins / metabolism* Phosphatidylethanolamines / metabolism* Thioglycolates / pharmacology |
| IF | 9.77 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC02975 |