論文 - 詳細
| RRC ID | 88458 |
|---|---|
| 著者 | Kulkarni VV, Anand A, Herr JB, Miranda C, Vogel MC, Maday S. |
| タイトル | Synaptic activity controls autophagic vacuole motility and function in dendrites. |
| ジャーナル | J Cell Biol |
| Abstract |
Macroautophagy (hereafter "autophagy") is a lysosomal degradation pathway that is important for learning and memory, suggesting critical roles for autophagy at the neuronal synapse. Little is known, however, about the molecular details of how autophagy is regulated with synaptic activity. Here, we used live-cell confocal microscopy to define the autophagy pathway in primary hippocampal neurons under various paradigms of synaptic activity. We found that synaptic activity regulates the motility of autophagic vacuoles (AVs) in dendrites. Stimulation of synaptic activity dampens AV motility, whereas silencing synaptic activity induces AV motility. Activity-dependent effects on dendritic AV motility are local and reversible. Importantly, these effects are compartment specific, occurring in dendrites and not in axons. Most strikingly, synaptic activity increases the presence of degradative autolysosomes in dendrites and not in axons. On the basis of our findings, we propose a model whereby synaptic activity locally controls AV dynamics and function within dendrites that may regulate the synaptic proteome. |
| 巻・号 | 220(6) |
| 公開日 | 2021-6-7 |
| DOI | 10.1083/jcb.202002084 |
| PII | 211926 |
| PMID | 33783472 |
| PMC | PMC8020715 |
| MeSH | Animals Autophagosomes / physiology Autophagy* Axons / physiology Cell Movement* Dendrites / physiology* Hippocampus / cytology Hippocampus / physiology* Lysosomes / physiology Mice Neurons / cytology Neurons / physiology* Rats Rats, Sprague-Dawley Synapses / physiology* Vacuoles / physiology* |
| IF | 8.811 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 205 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00806 |